XB-CLONE-3536797
Clone Name: IMAGE:7720434
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Gene:
etnppl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995174 [+]
cttgtgttgtaacaacgaaagaaattgcagaagcctttggggcaactggaatggaatactttaatacgtttggaggaaatcctgtatcctgcgcaattgg
actagctgtgctggatattattaaaaaagaagatctcagagggaatgcgaccagagtagggaattatttgactgagctactgaatgagcagaaacaaaag
cacccattgataggagacatcagaggagttggcttgtttgttggagtagatttggttaaggacagactgcttagaactcctgcaactgctgaggcccagc
atataatatataaattaaaagaaaagagaatcctactgagtgctgatggcccttacagaaatgttctgaaattcaagcctccaatgtgctttaccaagga
ggatgctaaactggttgtagatgagattgataaatgccttacagttcttgaaaaggcaatagaaggccaatcaaattctggtatacatgaaaaaaccgca
gccaagagaaaggtgcataatgaaaattcaggagacactaacacaaaagaagaggaaacatgtaccagcagcttacgagaaagcaacctaaatgacaatg
cctacagacaaagcaacggactgcatcctgggagccccaaagtcacacgtaaaagaataagaacataaaggaggtttctgagttcagaatcttcaaactg
ttgctgccttcctattctaacaactctaacaaatgaagatgcttggtctagtctggcgaagggttgttaaatttgatttgccaatgaattttaggggttt
atgtggngcatcctttttattttttttt
3' EST: CX995173 [+]gaggccagaatttaatgaataagcttttatttcttattatgttttgaatctagtttatagagaacaaatgatatactgtagatatgcatggcatggattt
agccaagcagtaggatctggtataatgtgattatggatatctgctcttctggcactctttcatagtcattcttgtcaagtgctgttatacatctgaaatg
ttctgacactttaagaaaagaagcatacgcatgtttacctagttttaagaaaactgctgatcccccatgtagcttctcattttaaaatataaaacattat
agcaccttgtatgttgacaaaaaagaaaataccataggcagtgagaaaatgcccccttatgctattctatggggctcatattcaagtaaaagggatatag
gaatcatgtacacagggatttcaggctgctacatcagatctgtagggacccctaataaaaagtaaaataatgtagcgagtggaaaagggttacttatttg
agcaagagctaaaatcttggtgtatctaaacagttggaaacggcttaaaattttcttgtacatagcatgctcagtacattcagcatgctgaggtctttta
aactgcatcttaaatctaagccattaaaacatgggaaaagattccattccaatatgatatactgaaactgcaaatacagaataaaaaaaaataaaaagga
tgcaccacataaacccctaaaattcattggcaaatcaaatttaacaacccttcgccagactagaccaagcatcttcatttgttagagttgttagaatagg
aaggcagncacagtttgaagattctgaactc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
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