XB-CLONE-3536779
Clone Name: IMAGE:7720340
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Gene:
apoa4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995186 [+]
agaagcattgataaagtgcatgcatatcaaccacaacgtaaaacattacagggccttatgttgttactggggaggttcatcccagacataatgaccaaga
aacaatggcagcagaattataaataaaatggaacattttagggctgtaagagaaacataaagctattcaggcacaacaaatgacagacataacacaaaga
caaatctgtgttttacaaaacttgctacagtgtgcatttacttcacttttacattttcttcttacttgatttgcagtaggccgacagcccaatctctggg
tatttttttttctttttatgattgatatggcatacaggtggtgcaagttgtaatggatactgtggtctgatgacaatttgggtgtatacgttgtttagtt
ttcagtttcagttatacctttatttatgaagtcgctaaacttatctcggatgtctttttctaagtattccatttgatcttgcacagtcactgtgtatttc
ccaaatttcagtttcatgtcctctaccctctgcactagtgccttgttgagtgcctctccatagggagtgatgctaactttaaattctgcaatcttctgct
ccatctgctggtttagctcggtgagatatggttctagtgtctgcttagcatccctagcatttacctggagtttctctttcagatcatctgcataggggct
cagttgagcttttaactgctcagtgatctgttttaattgggtgtctgcttgctcaacactctttctcatttggaattccatgctctctatctgcttggct
aaattctcctgcatctcctctgcataagggctcaggctctcacgt
3' EST: CX995185 [+]ttatgtgccatcacaggctcccaagccgatgtcagaacagaccaagcagtcagtgctttctgggactatgtctcccagctgcaaagcagcaccaaggata
cggtggaccagatccagcagtcagatatcaccaaacaacttaatgttctcatacaggataaaatgaaaagtatgaacctttatgctggagaactacaaag
ccaagtaattccctttgcaaagcaaatccatgaccaactaacacaagattcagagaaaattcgagagcagatacaacaggagctggagcagctcagggtg
aagatttctccttacgcagatgaagtacataaacaagtgaacaagaatttggaggagctgcagctcaaactgactccttatgctgaggaactgaaaagcc
aactagagaagaacactaacacaatcagtcagcaacttaggtctgtagttcaagaaatggattctaagattagggacaatgcaggaaatttgcaagaatc
tttggcaccatattcagaaaaactccaggcccaaatagatgagagtgtagaccaactaagaaaacaacttaacccttacactgatgaagtgagagataag
attggccatcaagtacaaaagttacgtgagagcctgagcccttatgcagaggagatgcaggagaatttagccaagcagatagagagcatggaattccaaa
tgagaaagagtgttgagcaagcagacacccaattaaaacagatcactgagcagttaaaagctcaactgagcccctatgcagatgatctgaaagagaaact
ccaggtaaatgctagggatgctaagcagacactagaaccatatctcaccgagctaaaccagcagatggagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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