XB-CLONE-3536731
Clone Name: IMAGE:7720344
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Gene:
coq6
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995218 [+]
ctatccctacttacaagccacagaataaaaaaaagtcaaagttaatttcagggttaactacctcttttaacattatgaatatgctaaggaaaaaaataga
ggatcattcccacggcactgtggaatgggcctttaatgtgactttttgtacaaccgaagtctgagaagctacaggcaacgattaacaagcaaaataaaaa
caaaaaaagatatttcagaggcatctgcagaaaataaatcaatatttatctttaatcaaaaatagtaaaccttgaattattttaatcagaaactgaatcc
aaattgatcttcttatttgcttgcaaaggccataatctgctcttttactggagtcagtgcatttgtagcttgcaggccaagcgtcctgagaagtacaatt
ggtggctgttttgtgttgtataatctctttagcagatcaactgcagccattaaaggaagattgtgcctctgtcgctcagtctcatattctagaaggtgct
ttgtagatcccaggtcacttccattgaatgcagcttgactcagatgatgggccaaacaggcaacatctccaaagcccatatttactccttgacctgccaa
agggtgcactctgtgtgcggcgtcaccaattaatgccactcgatgccggatatattcagtggcatgtttcagtcccagaggaaaggaagccctactgttc
tgctcaacacgtgacacactaggaggaagctgacgaggagaggagttagttggcatgaagaaagacaaagctgaatgaagtagagaaccagctgaagtta
tgaactctgaatgattttcgttgctccaaaatgctgaattcactgtatctacaaagctttcctcatccctgctgaccagctctgatgcatg
3' EST: CX995217 [+]gcgagaggctgctgtctagtctcaaggccgagctttgagctccacaggtcctgctgtatatgatgtggttatatctggtggtggtatggttggaactgca
atggcctgtgcattgggctcggatcctcacttgcagcacaagaaggtacttttgctagaagctgggaatagaaaaccatttgatcatctacctgagaact
tcagcaacagagtgagctccattaccccaggctcagctaccctacttgccagctttggtgcttgggatcatatcttggccatgaggctaaaaccatataa
gagaatgcaggtttgggatgcctgctcagatgcgctgatcacatttgataaggatgcattggaagatatgggatatattgtggaaaatgatataattatt
gaagcacttacaaagcagctggaattaatgtctgatcatgttgaggttatgtacagaagcagagctttgagctactcatggcctccaccatataataatg
gcaaagccacaccttgggtagagatagagctagcagatggacaaaggcttcacacaaaactcttgattggtgcagatggccacaactccatggtgcgcag
tgcagctggaatgcagagtgtccagtggaattataatcatgctgctgtagttgctacacttcatttgtctgaggcaacagataacaatgttgcctggcag
aggttccttcccactgggcccatcgcattactgcctctttctgacacatgtagttctttagtgtggtctacatctcctgaacatgcatcagagcttgtca
gcatggatgaggaaagctttgtagatacagtgaattcagcattttggagcaacgaanatcattcagagttcataacttcagctgggtctctaccncattc
agctttgtctttcttcatgcaactggctcctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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