XB-CLONE-3536719
Clone Name: IMAGE:7720536
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Gene:
scyl1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995226 [+]
cttattcacaataatgtctgtatgtcggcggtgtttgtggatcgagcaggtgagtggaagttggggggcctggattacatgtacacggctggagctgaag
acactgcacctctaaagggaattgaaatggagaagtacaaccccccagaaaagaccgaccgcagcaagacctccaaggagaagtggtcagctgacatgtg
gtgccttggctgcttgatctgggaggtgtttaatgggccacttcctcggccaactgctctccggtctctcggaaagattcccaaatcactggtgcctcat
tactgtgagctggtcggggccaaccccaaagtccgtcccaatcctgcacgcttccttcagaattgccgctctccggggggatttttttgcaacagttttg
tggagaccaacctcttcctggaagagatacagataaaggacccagcagaaaagcagacgttttttgaacagctcagtgaaaacttagactccttccctga
agatttctgtcgacataaaatccttccacagctgctgacagcatttgaatttggcagcgcaggcgctgttgttttgcccccacttttcaagattgggaaa
tttctgaatgctgatgaataccagcaaaaaatcattccagttgtagttaaaatgttttcctccactgaccgtgccatgaggatacgacttctgcagcaga
tggagaattttatccagtatttaaatgaaccaacagtcaatgcacagattttccctcacgtcgtacatggttttatggataccaaccctgctattcggga
gcagacagtaaagtccatgttgctctt
3' EST: CX995225 [+]aaaatttatacagcataaatattatgaaacctctgaccttgttctctcttcattatgggaagtgtcacagcttgctggggttgggtggccttgcacggag
tatgactctgagccataactggaccagtccgacaaacaatgaacatgatgtttctgctgtggagttaataataaaacatcaccaatgtgtcctttattta
tttcctagctcctctcagtccagtttcctcactcccagtttcaagggccctttggctgctctcctctcagcccttttggcttctatttctttttgacgct
cctctcgctttttccgggccatttcagctttactcagaccctgatctgcttccacggattcccagttgtcgtctcctccccaatccccggcgctatctga
attccgattatcatttttctggctggaaggctctgacaaagatgcaaagaagtccccttgtcggccgctgctgcctgaggtatcccagttatagtcgcta
gcaggccgagtaccttccagctgttgggatttagatgctgtgtgttccttagagggaggaacagtctggaatgcatcatcccatccccagccatcatcaa
gagcagaagttctgttaggagggggtgaaacctgctttgttttggaccttgaggaagaaaagtcaacagttttctcctgtgttttcccgtggccccagtc
tgtgtcccagtcgtcattctcagtctgacccctgttctgttcggcatcctctaggctgccccaatcttcatcatcccatcgatctaaggaattatccgga
gcacctttttcttcttcaggttcataactggttggggcagttgatgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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