XB-CLONE-3536645
Clone Name: IMAGE:7720639
|
Gene:
ddx21
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX995279 [+]
atgaaatggaggttgaaagtgtcaagaagaagaaacagtctgaaactcctctagccaagataaaaaagagaaaaatgcagaatggggcaacagaagatct
tgatttggaacaggtggcagaatctgtaaatggagagatcaacaacaataatccgactcccaagctgaagaaaaagaagaagcctgtgccacaatcagat
ctctctgaaaccgcagaacagtatgaaggagagcagccagatccaagcactcccataccaaagaaagtcaagaagaaaaaactaaaagagggcaaggaag
attcagacacacaggaagggacagacaaatccgaaccacaaatgaacggcgtaaagagtgttaaaaaatctaaaaagaacacaacaggtgatgataatga
accaactcctaagaaaagaaaaaaggatacaatggagatcacagcatccgaggagtgtgaagaaaagcagttaaccaaagaggaagaggaaataaatcaa
gaaaaaatagatggcgacttctctaaatttccaatttccaaggacaccattaagaatttgcaagctaaaggcgtgtcctacctgttcccaattcaatcca
aaacattccacactgcttacagtggcaaagatgtagtggtccaggcccgtacaggcacagggaaaacattttcctttgctattcctttagtggagcggct
taatgaggaccaacagccgttggctagaggacgtgcgcctagggtgataattcttactcccaccagagagttagctattcagatcacaaatgaaatccgc
agcatcactaanaagctgaaggtctgttgtttctac
3' EST: CX995278 [+]tttaataagggctaagcacacaggttttatatgcataaaaatatgtctcaagttactttaggaaggaatatttatgaacatggcagtatattaaaaaaag
ttattgtgacatgggatgttgggatgtttggagtcactaacggccccttctaaaccctccacttctatttctgcctcttcctcctcctcctcttcctcct
ctggaattatttcttctatcaaaaggtctcctccctcttcctccaaaacctccattccttggaccatcaaagtttctctcagattcttgaatcgcgggta
attcagttgcaactgtaaactgccaccgccttgtatctttccagccctcctgcattgactgcaggttttcagaacggacatcaaaacacacacccatgga
atccttcaggagacacattctatgaattttagaatcaatatcttcacccagttgttctttaattgatcgccatgcatagcttaagttgtgaatttgcact
gagctcttcaacgttattgtcacataacccgcttccatgttgagcaaagagcgttgtttaatggacgtcgccccagaaatatgagctaaagctgcagcta
gggcagttaatgcacccttcttctcaatcagctcctgggcgtattctttaaaatgctcaatcacatcagctggaactgaatccaaggactttattgcatc
agcactggatgatttggcaacattcaataaggaaggtattccaacacgtttgaatgtgattcccgtgctcctctccacattccgaagataatgcctttct
cttggttcataaagggatatgcagattcctgtgcgtccggctcttcctgtgcgccctgaccgatgaacataagcatctgcttccttttggtgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: