XB-CLONE-3536461
Clone Name: IMAGE:7720705
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Gene:
apoa4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995414 [+]
caggctcccaagccgatgtcagaacagaccaagcagtcagtgctttctgggactatgtctcccagctgcaaagcagcaccaaggatacggtggaccagat
ccagcagtcagatatcaccaaacaacttaatgttctcatacaggataaaatgaaaagtatgaacctttatgctggagaactacaaagccaagtaattccc
tttgcaaagcaaatccatgaccaactaacacaagattcagagaaaattcgagagcagatacaacaggagctggagcagctcagggtgaagatttctcctt
acgcagatgaagtacataaacaagtgaacaagaatttggaggagctgcagctcaaactgactccttatgctgaggaactgaaaagccaactagagaagaa
cactaacacaatcagtcagcaacttaggtctgtagttcaagaaatggattctaagattagggacaatgcaggaaatttgcaagaatctttggcaccatat
tcagaaaaactccaggcccaaatagatgagagtgtagaccaactaagaaaacaacttaacccttacactgatgaagtgagagataagattggccatcaag
tacaaaagttacgtgagagcctgagcccttatgcagaggagatgcaggagaatttagccaagcagatagagagcatggaattccaaatgagaaagagtgt
tgagcaagcagacacccaattaaaacagatcactgagcagttaaaagctcaactgagcccctatgcagatgatctgaaagagaaactccaggtaaatgct
agggatgctaagcagacactagaaccatatctcaccgagctaaaccagc
3' EST: CX995413 [+]ttgcattgctgggttgcattttattgagaagcattgataaagtgcatgcatatcaaccacaacgtaaaacattacagggccttatgttgttactggggag
gttcatcccagacataatgaccaagaaacaatggcagcagaattataaataaaatggaacattttagggctgtaagagaaacataaagctattcaggcac
aacaaatgacagacataacacaaagacaaatctgtgttttacaaaacttgctacagtgtgcatttacttcacttttacattttcttcttacttgatttgc
agtaggccgacagcccaatctctgggtatttttttttctttttatgattgatatggcatacaggtggtgcaagttgtaatggatactgtggtctgatgac
aatttgggtgtatacgttgtttagttttcagtttcagttatacctttatttatgaagtcgctaaacttatctcggatgtctttttctaagtattccattt
gatcttgcacagtcactgtgtatttcccaaatttcagtttcatgtcctctaccctctgcactagtgccttgttgagtgcctctccatagggagtgatgct
aactttaaattctgcaatcttctgctccatctgctggtttagctcggtgagatatggttctagtgtctgcttagcatccctagcatttacctggagtttc
tctttcagatcatctgcataggggctcagttgagcttttaactgctcagtgatctgttttaattgngtgtctgcttgctcaacactctttctcatttgga
attccatgctctctatctgcttggctaaattctcctgcatctcctctgcataagggctcaggctctcacgta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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