XB-CLONE-3536438
Clone Name: IMAGE:7720707
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Gene:
gpt
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995429 [+]
ccgaggcgggaaagttgctcgcaagatgtctgagaacgggacttgcaatcggatcctgaggctggagtccatgaacccctgtatccagaaggtggagtac
gcagtgaggggccctattgtcatcagggctgtggaactggagaaggagctgcagcagggggtgaagaagccattcacagaagtgatcaaagcaaatattg
gagatgcccacgcaatgggtcagaaacccatcactttcctgcgccaggtcagtgccatctgcctgtaccccgagcttatgaacgacaacaagtttccgga
ggacgtgaagcagaaggcagctcgaatcctgcaggcgtgcggcgggcacagcattggggcctacagcgccagccaaggaattgaagtgatccgtcaggat
gtggcaaagtacattgagaggagggatgggggcatccagtccgaccccaacaacatctacctctccacgggggccagtgactctatcgtgactatgctga
agctgctggtgtctgggcaggggaaatcccggacgggggtgctgatcccgatcccccagtaccccctgtattcggcagccctggcagagctaaatgctgt
gcaggttaattattatctggacgaagagaactgctgggccctggacatcaacgagctgcggagatcactgacagaagcccgaaagcactgtgaccccaaa
gtactgtgtatcattaacccaggaaaccccacagggcaggtgcagagcagaaagtgcattgaggacgtgatccattttgctgctgaagagaatcttttcc
tgatggctgatgaggtgtaccaagataatgtcta
3' EST: CX995428 [+]tttttttttcttccttggatctttattgattcatattaaaaacattcaggttagccctttatatgaaaatgctaaacaggactggccagtggtttggctg
cttggcgccttggctagaaggtccgttagggaaccggcggatctggcgacagtgagagatgactgagcagcagccaaggacgaccagacagccaagaata
cttaagactttggtgctaaattgccctagtgcagttgcccatagcaaccaatctgaataataaagtggagtgattggaatgggcaagtgcatgggtgcaa
ccagaacctgttactcagcgggtccttgggtgtttctgatgggttgataaaaatgcaaagctcatcctgaactttattttgcatccaacatggccgcctc
cggagaaagcacaagtgtaggcattccagtttgtcatcagagacattgcatagcaccttccatgcattcgtattatcactaaattccgagcagcgttggg
aactttgaggtgccctcctgggtacaatatcaggcaagcccaaacttagaagggacacttagggacttcagcagggcatcggagctggattaaggcctac
ggagctcagccacccaagggcctgaacgtaaagcttccatgatgctacattaatgggttaattatgtctaaaaagcaagagattaagcaacgagggccat
aaggggacccaataaggcctactgtactgcacccacattggtcaataaaacaccctaaaagcagagttacctctctacagctactagccagcagggccct
aga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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