XB-CLONE-3536384
Clone Name: IMAGE:7720713
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Gene:
apoa4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995464 [+]
ccatcacaggctcccaagccgatgtcagaacagaccaagcagtcagtgctttctgggactatgtctcccagctgcaaagcagcaccaaggatacggtgga
ccagatccagcagtcagatatcaccaaacaacttaatgttctcatacaggataaaatgaaaagtatgaacctttatgctggagaactacaaagccaagta
attccctttgcaaagcaaatccatgaccaactaacacaagattcagagaaaattcgagagcagatacaacaggagctggagcagctcagggtgaagattt
ctccttacgcagatgaagtacataaacaagtgaacaagaatttggaggagctgcagctcaaactgactccttatgctgaggaactgaaaagccaactaga
gaagaacactaacacaatcagtcagcaacttaggtctgtagttcaagaaatggattctaagattagggacaatgcaggaaatttgcaagaatctttggca
ccatattcagaaaaactccaggcccaaatagatgagagtgtagaccaactaagaaaacaacttaacccttacactgatgaagtgagagataagattggcc
atcaagtacaaaagttacgtgagagcctgagcccttatgcagaggagatgcaggagaatttagccaagcagatagagagcatggaattccaaatgagaaa
gagtgttgagcaagcagacacccaattaaaacagatcactgagcagttaanagctcaactgagcccctatgcagatgatctgaaagagaaactccaggta
aatgctagggatgctaagcagacactagaaccatatctcaccgagctaaaccagcagatggagc
3' EST: CX995463 [+]gattgctgggttgcattttattgagaagcattgataaagtgcatgcatatcaaccacaacgtaaaacattacagggccttatgttgttactggggaggtt
catcccagacataatgaccaagaaacaatggcagcagaattataaataaaatggaacattttagggctgtaagagaaacataaagctattcaggcacaac
aaatgacagacataacacaaagacaaatctgtgttttacaaaacttgctacagtgtgcatttacttcacttttacattttcttcttacttgatttgcagt
aggccgacagcccaatctctgggtatttttttttctttttatgattgatatggcatacaggtggtgcaagttgtaatggatactgtggtctgatgacaat
ttgggtgtatacgttgtttagttttcagtttcagttatacctttatttatgaagtcgctaaacttatctcggatgtctttttctaagtattccatttgat
cttgcacagtcactgtgtatttcccaaatttcagtttcatgtcctctaccctctgcactagtgccttgttgagtgcctctccatagggagtgatgctaac
tttaaattctgcaatcttctgctccatctgctggtttagctcggtgagatatggttctagtgtctgcttagcatccctagcatttacctggagtttctct
ttcagatcatctgcataggggctcagttgagcttttaactgctcagtgatctgttttaattgggtgtctgcttgctcaacactctttc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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