XB-CLONE-3536321
Clone Name: IMAGE:7720671
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Gene:
apob
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995507 [+]
tgcataatgcaaacaatgaaatgataatagttaaaaataatgccatcctataagaaacaatctttttattagcttacaataacgaaaacaaatttttgaa
ttgttcacacaaaaacacacatttgagggacgtgtaaaccggttgttgctactgtacttgcaaacaacacaaaagcctacgtttcagctatgccataata
tacagcctgctcagtttttttgccagttccgaatgttctcaatgatctgctctgcagttagtttcccttcttgaagttgtctctcgataaactgctgcag
ctcttcccatttctgagaacctttgtcgatgccatccagtaccatggcgcgcgcaatttctgctcgtttgcttataatgtcctctcttaaatgcgggatt
tcatcgaaggatttccagtcaaagggatgagggacttcaaccttaagttctcctttacgtgtggatatataggtcttcatttcttcagtggccgaattct
gaatgctttggagaagctggttgacatactctatgaaagagttgtagttttggatgataatgtcaatgagtcgacttgcctcggcaataagcttctcgta
tgaatttacaaacaggttagatgtagcttgaagtttttcattaagaatcttattggcattttgtgaaagttgcctggcagttgcagaccacttagttact
ctatcttcaagatctattgaagccctccttatgagttctttgccttgtccattagcattggtcagcaaatcgttagcatgttggtaatattttttaacaa
ag
3' EST: CX995506 [+]gccaaaatggccaacaatgcagatggttttgtggcttcaatcgatgcatctagcagttctacacgacgttttctggaatatgatcttgatgcatcagcaa
gcacagcaccaacaaatggacttgttctacaggggacatttgcacatactgatctaagcataaactggaaggacgatatcaactttaatggtttaaggat
tcctggacataacattgatatagacatcaaaagtccaacctttacagatgtccaaatacgtagtcaacaggaaaataacaaaataacttcatccgtgtca
tcttcatctgccggcactctggggcttgtacttgagaaggaatcctcagccctgaaagggaaactctttagtcggtccttgtcaacaccaggaaaagaca
cattaataatgaaaggtgaggtgtcccttaaaaatcctgaacgcattcagtacaaaatcaactggagagaagaagcagctatggatgtacttaatggttt
gaaagaaaggttaccaaagatttccaactccatccataattgtgtcaataaataccacaaggagcattttggaatggaaatcagtgaagcaggtctaaag
atgaaggaaaatctccaggataaaattgcatcaacctacagatctactgccaatgagatcaacaacatagaatatcagttgcagtcaacagccagaggag
ccagtgacaaatataatgaaatcgtcaacagtgcccaaaaatggtatcaagatccaaaggatatgtcaatgttcttgaatgatggtgaatataaagacaa
agccattgaattagtacgcgcctaccaaataagatgaaagacttgattgatgcagcta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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