XB-CLONE-3536044
Clone Name: IMAGE:7720604
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Gene:
ccdc125
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC167384 [+]
cgggtttccacgaagtacagggagcgcttagtaacgctgggctctcaggctgcggagctgctcggccgataggcaggaaatcacaagtggtggcagctgg
gagatcccgggcagctcaggtaattgcgttggactgcatttgcgtgtgactatgagcgatgcactttctgcagggagagtctgccattctgaggaggaag
aagactttatggccgggggagacttaggggaaggttttgggagaaagccgggaggcctatatgacggatcagtggatgccatggcagaaacatttaaaat
aagaagggtgtcagacaatcatggtcactatcagcttgtgaagaagggggaagattctggaacagcaagttgttctgtgttaagaaacaatgcctctgag
gccaagaccacccataagtttgggcatgtaggatactcacgatgcagaaagaacagtatagatgcaaatttggaagtgtccagtgaagatctgaggcagc
aggttcaagaattaacagaagaggtagattttttaagaatagaacttgaggcatcgcagaggcaacttaatagaaaagatgaagcactaaagctgttaca
aagcatggctatgcttgataaagccacaagtcacacaaaagaaatgcttcaaaaggctgagcaggaacggagaactttggaaaaggagatcagtgttttg
gaatgggaaattcagtttgatcaggtcagacttaaaaacatggaagaaacttggaaagaaaagtatgagcgagtgctctgtgaaaacgtgacaatgaaag
aaagtctagagttacggacaaatgaaattaaggctctgaaatctgagaatacaattctaaatcagcagtgccaggaacttcttgcaatgctcgatgtaaa
ccagcagaaaatatttcaggacaacctgtctctaaataaaaccagtttaatggatgtcacagcagttgaacttgcagtgcttggggcctgtacctgcggt
gatgggggaccctgtccctgtgccaaaatgtcttctgctactcgcaaacagctgcttcagctcaggaaggagcttgaagcagagaagaaaagtaaagagg
aagcatacattatggctgatggtttccgaattgcctttgagcagcagttgaagcgtagaaatgatgtgggtttgcagatagatgatatggagaaggtctg
taagaagggaaacaaaaggttacccaactggaagatccttaaagagaacggacacctgttttcaaaagaaggttacaaaggtttaggacaaaaattaaag
agcatgctaatttcacctgctgactgtaaaaatctagaatgcttagatgaaccccaagaaatcctcaagatactcatagatttgctaaacgataaagaag
aggcactagcacatcaacggaaagtcagctatatgcttgcacggactatggaagagaaggcagatccagcaacaagcccaaagagtgagactgacggaga
tctgcatgcagaaagttcacaacaaaacagttcagatacaagactgacaactgaccattgcacttgtcctacatatggcgattcatctgagagcaacata
tcattggaggagtctaaaaggattgttgaaaccgtattttcttcactgcacaaggaattatcacccaagcttgcctgtgtacaatcttccgagtcagctg
ctgagaatgaagcacagccagttggagtggggcacactgaaacatagaagaaatactagaactgtaaattaaaaaagcttacaattaaacattttttatg
tttgctgtacaattctgagagtgctgcttgccaaagaggcttacagtatatgcacctaataggcatagtgaactagttcggccagtatattgaaatatag
aaatatattttttttcaatgcaattttttcaaatacatttttaaaatgtaatatatatttttattctcattcaggtaagctcagatttgcaataacatga
ctcacaatgagcacctgttgcaacccagaagatgttcactgctgccaaaaccagatggtcagaatgaccatatggaggggaccagttagacggttataca
gctgcaatatgccagacctggtacaataagcccaatgcaaaagttacattgttggcagtctaatcttcagactttaactggaaaatagttacattaatat
attgtatataaattaaattatgttattttaaatgtctagagcagtgttcccatgttgctcaccaaccccttggatgttgctcccagtggcctcaaagcaa
gtgcttattttttatttctggttaggaggcaagttttggttgcataaaaacccagtgtaattgccaaacagagccttctgtaggctgccagtccacatag
gggctaccaaatagccaattatagctttcattggcaccctcaggaatctttgtcatgcttgtgttgctttccaacattttttccatttgaatgtggctca
cgggtataaaaggttggggatccctggcctacacagaagaacttcaatgcaaggtagaataaagagaaattcgctatttgcacattgctaagaataattt
agataagataatggacaatatgtttactcttgttcccagctgatgataaccttgatattttatatttctgttgtattttgatcagtagcaggttgtactt
atttaagcatttatgcttttcttatgtgcactttgtcaaggccatacatcttctatttttattatgtcatattttggtgttttgctgtaaaaataaatgt
ttctcaaccaaaaaaa
5' EST: CX995699 [+]cctcgattcaattcggccgaggcgggtttccacgaagtacagggagcgcttagtaacgctgggctctcaggctgaggagctgctcggccgataggcagga
aatcacaagtggtggcagctgggagatcccgggcagctcaggtaattgcgttggactgcatttgcgtgtgactatgagcgatgcactttctgcagggaga
gtctgccattctgaggaggaagaagactttatggccgggggagacttaggggaaggttttgggagaaagccgggaggcctatatgacggatcagtggatg
ccatggcagaaacatttaaaataagaagggtgtcagacaatcatggtcactatcagcttgtgaagaagggggaagattctggaacagcaagttgttctgt
gttaagaaacaatgcctctgaggccaagaccacccataagtttgggcatgtaggatactcacgatgcagaaagaacagtatagatgcaaatttggaagtg
tccagtgaagatctgaggcagcaggttcaagaattaacagaagaggtagattttttaagaatagaacttgaggcatcgcagaggcaacttaatagaaaag
atgaagcactaaagctgttacaaagcatggctatgcttgataaagccacaagtcacacaaaagaaatgcttcaaaaggctgagcaggaacggagaacttt
ggaaaaggagatcagtgttttggaatgggaaattcagtttgatcaggtcagacttaaaaacatggaagaaact
3' EST: CX995698 [+]tttttttggttgagaacatttatttttacagcaaaacaccaaaatatgacataataaaaatagaagatgtatggccttgacaaagtgcacataagaaaag
cataaatgcttaaataagtacaacctgctactgatcaaaatacaacagaaatataaaatatcaaggttatcatcagctgggaacaagagtaaacatattg
tccattatcttatctaaattattcttagcaatgtgcaaatagcgaatttctctttattctaccttgcattgaagttcttctgtgtaggccagggatcccc
aaccttttatacccgtgagccacattcaaatggaaaaaatgttggaaagcaacacaagcatgacaaagattcctgagggtgccaatgaaagctataattg
gctatttggtagcccctatgtggactggcagcctacagaaggctctgtttggcaattacactgggtttttatgcaaccaaaacttgcctcctaaccagaa
ataaaaaataagcacttgctttgaggccactgggagcaacatccaaggggttggtgagcaacatgggaacactgctctagacatttaaaataacataatt
taatttatatacaatatattaatgtaactattttccagttaaagtctgaagattagactgccaacaatgtaacttttgcattgngcttattgtaccaggt
ctggcatattgcagctgtataaccgtctaactggtcccctccatatggtcattctgaccatctggttttggcagcagtgaacatcttctgggttgca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: