XB-CLONE-3536023
Clone Name: IMAGE:7720606
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Gene:
tlk2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995714
[+]
atgatcttgacttctacttgaagcagcacaagctaatgacagaaaaggaagctcgctctatcatcatgcagattgtaaatgcccttaagtacctgaatga
aatcaaaccgcccattatacattatgacctgaagccaggtaatattctgttagtcaacggtactgcttgtggggagattaaaatcacagattttggtctt
tcaaaaataatggatgatgacagttataattcagttgatggaatggagcttacatcacaaggagctggaacctactggtatctgcctcctgaatgttttg
tagttggaaaagaacctccaaaaatctcaaacaaagtggatgtatggtctgtaggagtcattttctaccagtgtttgtatggtcggaagccctttggaca
caaccagtcccagcaagatatcctgcaagaaaacaccatcttaaaagccacagaggtgcaatttcctcccaagccagttgtgacccctgaagccaaggct
tttatccgtcgatgcctcgcctacaggaaagaagatcgaatagatgtgcagcagcttgcttgtgatccttacctactgccacacatccgcaaatcagtct
ctacaagtatcccagccaacgctgctgtagcgtctacatctggttcctccaataatagcttgtcaaactgagaacccagcattgcattaaagaacagtta
ttgcgacaagcagaaggttcagtcatttggtggcttctggagattttttttctcccttttcgtggagaagtagtgcattttcaggacaaaaaaaatgaac
ttgagtttgggcttttt
3' EST: CX995713
[+]gaggcctttaatggagatgtttaaacaaaatacttctgacctcaggaaagagcttcttaacttcttagtgatgattagggtgaaggtggtattgtgttta
tccaatcttgaaactgtttacttgagtctttaagaaaaatggatgaaggggggggaaacagaacaggtttaaatatcaacggattctctagtacagtttc
cactcctggaaaaactgctttatgtaagtcagttctctggactgaagggtgttcaatttgagtgtgaagaaaaacagcagtctggcattgaggaaggaaa
ggtttaggtccagctcacagaccaagtctcatctgcacactcctctccttattgtgaaagcactttaaaagaatgtttagaattcctgcagcggttcttt
cacaagaccccaaaattcaatccagctgtatgtggctctaaaaagccaaactcaagttcattttttttgtcctgaaaatgcactacttctccacgaaaag
ggagaaaaaaaatctccagaagccaccaaatgactgaaccttctgcttgtcgcaataactgttctttaatgcaatgctgggttctcagtttgacaagcta
ttattggaggaaccagatgtagacgctacagcagcgttggctgggatacttgtagagactgatttgcggatgtgtggcagtaggtaaggatcacaagcaa
gctgctgcacatctattcgatcttctttcctgtaggcgaggcatcgacggataaaagccttggcttcaggggtcacaactggcttgggaggaaattgcac
ctctgtggctttta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: