XB-CLONE-3535999
Clone Name: IMAGE:7720752
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Gene:
zfp160
,
znf287
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995734 [+]
tgaagattaagaatacattaaccaaaaggaatctgataacatcataatcgtatgtgactcactgtgtatcagtacagttactgtcaggcaatggttcctc
ccctgtgtgtaactgggaatgtgcagtgagctgataacgatacataaaacatttcccacattcatcgcaagcaaatggtttctcccctatgtgaattctt
gtatggaatttaaggcccgcgcgatcacggaaacatttcccacattcactacaactgaatggtttctcccctgtgtgaattcttgtatggaatgtaagac
ttatgcgatcacggaaacatttcccgcattcagtacaactgaatggtttctctcctgtgtgaatttttttatggtatctaagggctgcgccatcagagaa
acatttcccacattcagtgcaagtgaacggtttctcccctgtgtgaattcttttgtgtacagtaaggtatgaggaatacttaaaacatttcccacactca
gaacaagagtacggtctctctactgagtgaaccctaagatgtacattaagcagtgaggtagacatgaaatgtttcccacattcagaacaagaatatggtc
cctttcccacgtgactactttgatgcacaataagctctgagtgagacgtgaactgttttttacattgggagcaaatacataatttttctagtctgtgaat
tctgcagtgtctgtaaaagtctgaactgtgtgaaaaacgtttcccacagtcagtacaggaatagggtttctctcctgtgtgggttctgtgatgctcaata
agatgtacatgccgggtaaaccttttcccacattc
3' EST: CX995733 [+]agccaatgggaatgtgggaggaagcgagtgacccagtgatggggaagaaggagaaaaatgaggagcggaaggagagaatcctgaatctcacactggagat
tatctatctgctgactggagaggtgaggggggcactgtgagtggcacagtgagaagagactgtctgtctgtacaaagggggtctctctgctgcgttacac
actcatcattctctctccctctcaatacatagggctacgtcatccctaagaagaagagcccaactgtgggtttgggggccctgcatgcccctggctccgt
catacagaaggaaaatgacaagaagatcctggaactcatctccaacatcatccagctgctgactggagaggtgggtgcggccccccaatccccccttatt
gtttagtaacagtgtatgataatccctttctctggctgggggatgactgtaacattgtgtgtgccaggttgccataaggtgtgaggatgtttccatctat
ttttccttggaggagtgggagtatataaaaggaaaccaggcccttctcagaaaaattatgaaggaggaggaggagccccagcaagtctgcccaccgggta
agtggcagaaatggaccagacagatccagttgatctcctaagtgtcgttggcattggagagcaatcaaatacatttggttggtcagtctgactcctgtgc
cagtatcattcctgctcttaattatggtttgagtcacagtgagattggactcagcgccacattaaggatttatatgtgacttttcatcccgtatcagagc
ctgtcggggtgacagttcctgcgctgctgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: