XB-CLONE-3535974
Clone Name: IMAGE:7720802
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Gene:
tubd1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995752
[+]
aaaaaatataacatttattgccaatatacctatttacattttaatgcactttaagtacagctacatttaacacacagcattgaatgataaaccattcagt
ctttgaagtaaaaaaaataataagaacatgattataaattggtgtaacttgcaataatttgctcaagagttgtgaaagtttccaagaattcttcttcgtc
aatcccaaattttgtgtattgatggacatatgccctaaaaaaaaataaaagaagaactttacaatttaaattgttgtttgatttccagatctatctcttt
aaattaaaaatgtttatttctacctcctcctctctacattaaagtataatattcagataaaacttctgctttaaaggggctggccattagatgctgtttt
agaaaatgcctgagagttactaatgatgcccaaagcttcacctcaagttctgtttggtgaaaggagattttagataatgaaatactctatcatctttcat
tcttcatgttggccagatgactgataattatttctgtcatcttactgtggctcttacaccttctcaattggttctatctagcttactgttccatcaagta
agcctgaccacaccactttataaaaatggtcaaatgtcacaagttatacgcacatgctttagaacaatatggggtatattctgaaattgttagcattgtt
atattcaagacatactttgatgcaaacatgttccatgcttttccaacaatattatccaagggtttcagcagaaactggctattgctaaccaaagtagcag
acttctcatatttgttgaaagctct
3' EST: CX995751
[+]gctttgagtatagttttactttagggcactgtgcttccagttataaaattagtttttatccacaagagattataaactactttacaccttcatatatact
ataatatataatataatactttacaccttcatatatactataatatatactataatggttgattttataaaaaaaaaaaaataattatatacaagttgct
caagatctcatgttattgtaggtttaatgtatttgtgccaaatatctgacttagcagtatgatttttcacaagttacatttgtgtagtatagtaaactat
aaccacattttccaactaatatacagagtatgctattttaggtattaattggcatgtgcggcccccatctcagccaacagcagaaaagacttccctgaga
aaagaattgcaatttaatacttctgttgctaacttggcaattcttcgtggaaaagatgtgcaaactgcctctttaggtaagttaaaacactcttctttgg
tgctttacagtactagtgtaagtgcttgaatatgccatatacatatttgaatttccattaaaaagaaaatgattgttctatattttaatttgtcttgtct
attttttttttgtaagaccttgttaccccccacattttcagtttgcccaagaacatcacaccaaagaaacaatgctgttataaagttacacattttactg
aaccgatgaattgcttctgccagtccgtttgagatgaggtttataagtgcatattatggtataaagattttatggaattccgaatgcttcacagagagat
cttcaaacagttgtacccccaacaatagtctgatatgaagcatgtgcagagactgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: