XB-CLONE-3535916
Clone Name: IMAGE:7720808
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Gene:
ubxn6
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995797 [+]
tcggccgagggagaaatcagctgtctagagggttcccatgaattctttgaggctgttggctttgaaaagaagacaatgcctgggcaagatttacaagaag
aattttttgttctgggtggtgatgcactgaaaaacctagatgcattacatgggcattgtgatgcattgctgagtgcagagcctttgagactaagtctgga
gaggcaattgcgtattttcatgccatccattgaagctgcacactttgaccttcctgatgactttttcaacctgactgcagaagagataaagagggaacaa
agagaaaggacagaaatggtggagaggaatgcagtgctcagaaccaaagcaatgcgagagcgtgaagaacagagagaaatgaagaaatacaattacactg
tgctgagaatcaaattgccagatggatacatcttgcagggcatattctttgctcgggaacgggtgtctgctttgtttgattttcttcgtgagcagctgca
aaatgattggcttccttttgaacttctggctccagggggtcacaaagtagagaatgagcaggctacattgatagagtgtgggctggtaccatctgcacta
ctgacctttcgctgggatgaggctgtcatggctgatgtggaggctgggggagggcagagttcagtcagtgtaataaagccagagcttctctcanaggtag
agcaactcacataggaaatatcactacatgtcccgcctgatgatattcattttggaaagaaaacaaaaaagtgcttgcctggagtcattagttttcaagg
catgttgcatttcagatacaggtaggcaatcaatcagcagggaacat
3' EST: CX995796 [+]ttttatgcaaatacctttttattcaaaacaaatcctctagatcaatagagtttaatggagaaagacatttgttgcgaaatacattgccttatcataacta
aactgtttcttgcttattggtttatctgaaaagctgctttggtacagatcctggtcttttatttaaccaaaggttaacctataacctgtgggcagttgtg
gctaaaaacaaaaccataattgaatgttccctgctgattgattgcctacctgtatctgaaatgcaacatgccttgaaaactaatgactccaggcaagcac
ttttttgttttctttccaaaatgaatatcatcaggcgggacatgtagtgatatttcctatgtgagttgctctacctttgagagaagctctggctttatta
cactgactgaactctgccctcccccagcctccacatcagccatgacagcctcatcccagcgaaaggtcagtagtgcagatggtaccagcccacactctat
caatgtagcctgctcattctctactttgtgaccccctggagccagaagttcaaaaggaagccaatcattttgcagctgctcacgaagaaaatcaaacaaa
gcagacacccgttcccgagcaaagaatatgccctgcaagatgtatccatctggcaatttgattctcagcacagtgtaattgtatttcttcatttctctct
gttcttcacgctctcgcattgctttggttctgagcactgcattcctctccaccatttctgtcctttctctttgttccctctttatctcttctgcagtcag
gttgaaaaagtcatcaggaaggtcaaagtgtgcagcttcaatggatggcatgaaaata
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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