XB-CLONE-3535850
Clone Name: IMAGE:7720814
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Gene:
ahnak
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995845 [+]
acaaatttaaaatgccttcagtgcatgtgcctgaagtgtccatgcctgatttcggcttaaatgtaaaagggcctaaagtagagggagaactgaaaggacc
caaaattgatatgaaagggccagaggttgatgtagcaattccagatgttgagcttgaggggccagaaggaaaactgaaaatgcctaaatttaaaatgcct
acgtttgggttttctggctcaaaagtgaagggtaaagatatagatattgcacttccagaaggagaaatagacgtatccgtaccaaagctggacatcagtg
ccccagagttagacatagaatgcccagagggaaaagtgaaaggtcccaattttaaaatgccatcactgcatatgcctgatatagacttcaatcttaaagg
tcctaaagtggaaggtgatttaaaaggccccaaagttgacataagtgctccagatgtagatattgatggaaaggtgaaaggtcccaaagttgagatgcct
tcggtgaatctagccacgcatgatgtacacttaaatttgaaaggccctaaactagaaggtcaaggcattgacccaaaaggtccagaaggaaacattgaca
ttgctattcctaaaacaggattagacattaaggcaccagatgtcagtcttccaagtacaggattggatattgagggccctgaaggaaattttaaaatgcc
taaggtaaagaaaccaaaatttggttttggaatgaaatcccctaaagcagatattaatgtaccagcagtagatatcggcacaccagatgttgaaagtaat
gcagaaatgcctgaaataggtgttgcagg
3' EST: CX995844 [+]ttgctcagaaacaatccaccgcttcacacttcttaatgctttggacattaaagtgcttacagatttttgaagttaaaatgtttttttttccttgtgtttt
ttgttttattaacagaaatgtaagctaaggttacttctcaccaccccctaaataatgaagaacataaacaagcggcagttgagatagttgatgacaaaaa
tatcacaaataacaaattgagccagactgggaaagggctaagcagattgaaaatatttaggcattatgagctttagcattgggacacatcacagtgtctc
ccataaaatgtgtaaaccttaggttaatggaccatggccagtcaaggagcctcttctctcctgctgcagattcccattcatataaatggtaaacaagctg
ccaaatgcaaatactggaactggcaccttggcattactaagcaatccttgcctgacagcatacatagcttgtttcctattcacatgaaccatgtgccatt
aacctaagtgcaacatattttaagcagcagtaatagtctgagatcattacagaacttttatgttcatgttggtatgttttaaatggaacctacattttaa
atagtcaccatctaacattcaaatgtatatatgtctgcaacaacagttaatacggctgctttacattctgctttgattctttcccagtggctcactcaga
gatacagtgtcttcaataaatcagattgtatgtaatctgtataggcccttaaagaaaataaagctttaaaagcaagttaaaaaatatggaaaaatatatt
tccatgtacaaacttataacanaataatcaatggtctcacctgcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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