XB-CLONE-3535826
Clone Name: IMAGE:7720864
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Gene:
pxdn
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995860 [+]
gggtcgtgaccatggcatacctccttaccatgattttagagtcttctgtaaggtctccacagtgcagacatttgacgatcttagaaatgaaataaagaat
ccagatgtcagggagaagcttaagaggttatatgggtcgccattaaacattgatttattccctgccctgatggttgaagacctcattccaggaagtcggc
tggggccaacattaatgtgccttttaacaacgcaattcagaaacatcagggatggggacaggttttggtatgagaatcctggtgtattcactgcagccca
gctcacacaaattaagcagacctccctggccagagtgctatgtgacaatggagacaacatcaccaaagtgcaacacgacctgttcagagtggctgaattc
ccacatggttatgtcagttgtaaaaacattgctaagatggacctaagggtttggcaagattgctgtgaagattgtagaaccagggggcagttttcgacat
tctcaaatcacttcagagggaaacgatctacagagcacagttataaggaggataataaagaaccatctagcttgttgaatcagtctgtaaataccacttg
taacactgaacagccaaagaatctgccacacgtgaatgactttaaagaatttgttttggatatgcaaaagactataacaggtcttagaaaacagatcaaa
aaacttgaatctcgcttaagcaatacagactgcactgatgaaactggggaatcccattctacaaaggagaaatggaacaaagatgcctgtacaaagtgtg
aatgctataacgaacacattacctgc
3' EST: CX995859 [+]ctatagggcgagaggttaaaacgtaggcctatttatttaagttgcattcatttacgaagcattcattacacagtgcaaagaattctttggtaatattcct
gtttacaatacaaaagactaaggcactcattttggtatgaaagttctgacaataaatatgcaacatttgcagccctggagagaaagaaaggaaggacctc
agagtggaaaaagaacattactgactgctgtagaaagaaaaacatcagctggggaaatgactcagttgtttatacaagaatttatctgtgccacacaact
ctctgccagtgaagtcattgtctaaaaggtcccaaagtctctctggatcatgactaagcacagagaatgtgtgccaattattatttccattcacaatgta
gtcggtggtaatacatccatacagagtattaatgattaaagaaccattgcacccaaaatgatgcagaacactcagcacctagtatagagcgaaagtctgt
accagtaaagtatatagatgagaaaaggaaggaatttatctctgctgctgggtattcgcagctttgtttggttggtcaaatcccttttgacaagagcagg
gctgtgggactgataaagaatcatcgtggctggtcagttagaaacaatattaacttagtaaagacttcatttgcaagtggagcattaacacacaactaat
atgaaaaattaggacacaatggcgatataaatactattttggctgcaatcggctcttatttcaatggtgaactattgctcagacagttaatgatgagcaa
atctgtcccattttgctttgctgaaaactttgcgcaaacttggcaaaaagttgcaccaaattaggtttcacagtgtttttttccctgtggaacctaatga
aaaattgtgctcatcgctacgttc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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