XB-CLONE-3535764
Clone Name: IMAGE:7720870
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Gene:
etnppl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995902 [+]
atcgattcgatttgatgttatgtagtaccattaatacttgacattgatttctgttgtctaatatttaaaaactatgactgaaacactcccaatctgtgac
tttcagtttcaccaatttcctatgccaaacactcaaatatctagatttaatggtcaagaacaacaaaatccaaataactgcaagactggataaaaacatc
taaaacacacagaaacaaatctagtttatttgactagatgtgcagaatttaatgaataagcttttatttcttattatgttttgaatctagtttatagaga
acaaatgatatactgtagatatgcatggcatggatttagccaagcagtaggatctggtataatgtgattatggatatctgctcttctggcactctttcat
agtcattcttgtcaagtgctgttatacatctgaaatgttctgacactttaagaaaagaagcatacgcatgtttacctagttttaagaaaactgctgatcc
cccatgtagcttctcattttaaaatataaaacattatagcaccttgtatgttgacaaaaaagaaaataccataggcagtgagaaaatgcccccttatgct
attctatggggctcatattcaagtaaaagggatataggaatcatgtacacagggatttcaggctgctacatcagatctgtagggacccctaataaaaagt
aaaataatgtagcgagtggaaaagggttacttatttgagcaagagctaaaatcttggtgtatctaaacagttggaaacggcttaaaattttcttgtacat
agcatgctcagtacattcagcatgctgaggtcttttaaactgcatcttaaatcta
3' EST: CX995901 [+]tttgtactgaccacaggaggataaacttagatctgatatcttcttttatatagattgctgcgtttattgctgagtccatgcagagttgtggaggtcaaat
aatcccaccagcaggctacttccagaaagtgtcagagtatgtacagagcctaatgagaaataatggcttttctcggttcacttaatctagcttttctatc
ttatagcacattaaactcatacagtccaaacaattacaaaacatataggagctgaattactaacagaagttctaaactgcactagagcagttgcaaatag
cagccaatcagcaatttgttttaatcagtctactacaagttagaatatgaaagcaaaggtctggtgatatttagcttctttgttagtaagtcagccccat
aattgtgacactattgtgagattatactattcctacagaatgcaatacaaacttggtgtagacatttattgtatacaaacacaatgttaagaagaagaaa
ctttccaatcattcattgtcttttttatgtttaccttcattctcttgactagtttctatacatctttctaccaaccttttcagaattcctatgtttcctc
tctctctctctctctctgtttttatatcatcatcattgtctggaaataggtctgcaaagctcattcactaatatgtgcaagataccatataagtctagtt
tcacacttctaaagatgtgtaaaaagtgaatttctgccttttattccatagtgtgcatctaatctgagcagtttgtcacacctacacttacatgtgtcac
atgagtttcactacttatcttaataagatctatacgtgtacagt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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