XB-CLONE-3535696
Clone Name: IMAGE:7721021
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Gene:
rars1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995946 [+]
tgtaagtgagacaccaagcttagagggacttagggaagagaacgccaagctcaaatatcggctgaacattctgcgtaagagccttttggaggaaaagaag
aaatcatcaaaaagcatgataaatatcaatgcgcagatccaagagatctttggcacagctataggagctgcctatcccgaactgcagaatgctcctttgg
cagtcacaccaagccagcagccaaaatttggggattaccagtgcaatagtgctatggccataactcagatgctaaaagctatgaatcaaaaagtgagtcc
cagagaaattgccgacaaaattgttaaaaacattcccacaaatgaattggtagaaaaagtggacatcgctggtccaggttttattaatgtgcatctacat
aaagattttacatcaaaacagatctccaaactgcttgtaaatggggtccagccccctgtaatttgggaacggaaaaaggttgtggtggacttttcttctc
caaacattgccaaagaaatgcatgtaggccacctgcgctctacagttattggtgatagtatatgtcgcctttttgaatttgtgggccatgatgtactgag
actgaaccacttaggagactgggggactcagtttggaatgctaattgctcatctccaggataaatttccagattatttgaccgtctcgccacccatagga
gatctccagtctttttataaggaatcaaaaaagaggtttgatgaagatgaag
3' EST: CX995945 [+]cagtaaatctttttatttgtggagttttagaaaagtaaggttctgtcatgttgtatgggccctgaattacattttctgtacaggtttgatgcccagaata
tcaaaacctttggccataacagcagcggtggcatcacaaagcaacagccggctcatgttcaccttaacgatctgaccagtttgccgatccttttccacac
agtaacagttatcatagaactcagtgaaggtggtagccagttcatacaggtagtcacacagagtgtggagcaggaggtcatccaagatcttctgcagtat
ctcaggaaaccttagaatgcatttgcttagcttccactccttttcatgctctaaaatgatctctgtttctttggcagctttgtgcaagtcctcatcactg
atattcgccaaccttgctatggaccttattctggtgtaggcgtaaagaaggtaagcagctgtattccctcgatcatccaacatcttgtcaaatgagaaga
tgtagtcattcattctgttatgagacaagtctgcatactttatgcagccaaaggcaacagatgtttgagcagctagtaattcctcagcagttaagacctt
atcccttcctttatctttaagcttttccattgaccgcttaagcccttcatccaacaaatctattaagcgcactgtgtcaccagatctggtcttaaatttc
ttcttgtcttctccaaggaccacaccaaagccagcatgttcaattctagttactttgggatcgtaccaaccaatcattcggcctgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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