XB-CLONE-3535667
Clone Name: IMAGE:7721263
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Gene:
apoe
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995968 [+]
tgaacatgtacatgaagaaactgaagaaacggctgagtaaagacagtgaggatttaaaaaagaagctcagccaatatggagaagatttccgtaagagtac
tgaagacaaagtggaaaatctccgtaaagccgtggagccttatttgtccaacgtgaaagataaagggcagcagaggctgatgaacctgaaggatgccata
gatgagcaagggaaacaactcagagaccggtttgtctctgttgccaaagacgcccaagctaaaatgaagaagacagcctctgatgtgaaaagcagtgtgg
ataaactaggggaacagttccgtaaatggttcaaccccaatgtagagagcctgcgcaatcagcttcaagccttggctaagagcctgcagaccaaggccaa
caaataaatgaaagaaccagtgtttttccttgcttctcacttgcagctgctacttttgaccataagattgcgccctgaggtggagcaggacattaaggat
ccaacatgcctaaacatcttgcctttccctgtttgtatctgaggtacatcacccagtgattatactgctcacctaggcaagctcattaatcagatctctg
acattaatgacaagcacaaattagaggtgaacgatgtaaagccaaagggaacttcagtattaaaggcacatttaacctaagggcaagccttaaattatag
cactaaactatccccagcatattctcctgcactaaacatgccttgtgtcctatggatctccagctgactgccctgtctgaacaagaacctacatacctta
ggcaaagttagatttcacagaaagctaaga
3' EST: CX995967 [+]atttggataaaaattgcattgaaatagacatatattttacagcagagaggcactttataaagtaggtaaggcagcacatacagcagaaatttagtgaagg
cacaacgttattttaggtttaaaaaaaggggagtgtattaagaaacataaagacaccagcaatgcccataatggtgccctacattttcaagagctggtga
tacaatatggatatttagtaggttgagatacagacatccatgaaccctcttgtttaagaccccagctgtttggtacaagtgattccttgcatggcctagt
ttactgcttgcccttggggcctgtgttccaaatggaaaatataacatgtagaggctcatatacttttctgtcttagcttttctgtgaatcttaacttgcc
taaggtatgtaggttcttgttcagacagggcagtcagctggagatccataggacacaaggcatgtttagtgcaggagaatatgctggggatagtttagtg
ctataatttaaggcttgcccttaggttaaatgtgcctttaatactgaagttccctttggctttacatcgttcacctctaatttgtgcttgtcattaatgt
cagagatctgattaatgagcttgcctaggtgagcagtataatcactgggtgatgtacctcagatacaaacagggaaaggcaagatgtttaggcatgttgg
atccttaatgtcctgctccacctcagggcgcaatcttatggtcaaaagtagcagctgcaagtgagaagcaaggaaaaacactnggtctttcatttatttg
ttgggccttggtctgcaggctctt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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