XB-CLONE-3535652
Clone Name: IMAGE:7721121
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Gene:
srsf6
,
smim3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC154892 [+]
GGCAGACTGTACTAACATGGTGCCTGGGCTGTGTGCGGCCATTCCTGAGTAAAGCCGCTCCTTTTCACTTCTACCATCAATGGAGCAACTTTGAGAAGGA
ACAGCTCTGTTGATTGAAAACCAAAATGAGTGACCTTAGCACACCCTCGCCAGTGGAACTTCCCAAGCATATCCTGGATGTCTGGGTCATTGTCCTCATC
ATACTGGCCACCATTTTGGTCATGACCTCCTTGTTGCTCTTTCCAGCAGCAGCTGTTGTTATGTACAGAGTACGGACACACCCAATCCGAAGTACCAGGT
AGCGGTGCAGGAACAGGATCAGTTTATTTACAGATTTGTAGATTTGGACTAACAATGGACAAGAGACAAATCTTCGTCCCACAAGAAAGAACTGCAGTCT
CATTTTCAGCCTTTATTGAAGAATGGATGTGCAAAGAGACTGAATATGTTGGGGGGGTTGGATGATGAAATGTCACCCCTCCTGCATTAAAATATCACTA
GAAATCAAATCACTTATTTTATGCCAATCACAGAGAGAAAATTGTTTTCCAGTTTGAACGGATTGTAATGTATTCTTTCAGCCTTCTCTTGTGAAAGATG
AAGAAGTAAAGAGAGTGAATACATTTTCTTTTTCCAGCTCCAGATAACCAGCTCCTGTAATTCCATATGTCTCATTTCAAGTGAAGGTGCCCATTGCAAC
CAGTTAGCCATTAGTTTTGCTGAAACTGAGGGGCAACTGCCCTGGTGCAAATGAGCCCCGGTGTCTGTTATAAGAGAAAGCTGCTCCTGATCTGTTATTG
CCATACTAATGTAAAATACATCAAAACTACAATGGAAAAGTTTGTGTGAAAGCATTTAAATAAAAATCCATTTATGTTTCAAAAAAAAAAAAAAAAAA
5' EST: CX995978 [+]gcagactgtactaacatggtgcctgggctgtgtgcggccattcctgagtaaagccgctccttttcacttctaccatcaatggagcaactttgagaaggaa
cagctctgttgattgaaaaccaaaatgagtgaccttagcacaccctcgccagtggaacttcccaagcatatcctggatgtctgggtcattgtcctcatca
tactggccaccattttggtcatgacctccttgttgctctttccagcagcagctgttgttatgtacagagtacggacacacccaatccgaagtaccaggta
gcggtgcaggaacaggatcagtttatttacagatttgtagatttggactaacaatggacaagagacaaatcttcgtcccacaagaaagaactgcagtctc
attttcagcctttattgaagaatggatgtgcaaagagactgaatatgttgggggggttggatgatgaaatgtcacccctcctgcattaaaatatcactag
aaatcaaatcacttattttatgccaatcacagagagaaaattgttttccagtttgaacggattgtaatgtattctttcagccttctcttgtgaaagatga
agaagtaaagagagtgaatacattttctttttccagctccagataaccagctcctgtaattccatatgtctcatttcaagtgaaggtgcccattgcaacc
agttagccattagttttgctgaaactgaggggcaactgccctggtgcaaatgagccccggtgtctgttataagagaaagctgctcctgatctgttattgc
catactaatgtaaaatacatcaaaactacaatggaaaagtttgtgtgaaag
3' EST: CX995977 [+]gaaacataaatggatttttatttaaatgctttcacacaaacttttccattgtagttttgatgtattttacattagtatggcaataacagatcaggagcag
ctttctcttataacagacaccggggctcatttgcaccagggcagttgcccctcagtttcagcaaaactaatggctaactggttgcaatgggcaccttcac
ttgaaatgagacatatggaattacaggagctggttatctggagctggaaaaagaaaatgtattcactctctttacttcttcatctttcacaagagaaggc
tgaaagaatacattacaatccgttcaaactggaaaacaattttctctctgtgattggcataaaataagtgatttgatttctagtgatattttaatgcagg
aggggtgacatttcatcatccaacccccccaacatattcagtctctttgcacatccattcttcaataaaggctgaaaatgagactgcagttctttcttgt
gggacgaagatttgtctcttgtccattgttagtccaaatctacaaatctgtaaataaactgatcctgttcctgcaccgctacctggtacttcggattggg
tgtgtccgtactctgtacataacaacagctgctgctggaaagagcaacaaggaggtcatgaccaaaatggtggccagtatgatgaggacaatgacccaga
catccaggatatgcttgggaagttccactggcgagggtgtgctaaggtcactcattttggttttcaatcaacagagctgttccttctcaaagttgctcca
ttgatggtagaagtg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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