XB-CLONE-3535646
Clone Name: IMAGE:7721217
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Gene:
apoe
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995982 [+]
ttaaaaaagaagctcagccaatatggagaagatttccgtaagagtactgaagacaaagtggaaaatctccgtaaagccgtggagccttatttgtccaacg
tgaaagataaagggcagcagaggctgatgaacctgaaggatgccatagatgagcaagggaaacaactcagagaccggtttgtctctgttgccaaagacgc
ccaagctaaaatgaagaagacagcctctgatgtgaaaagcagtgtggataaactaggggaacagttccgtaaatggttcaaccccaatgtagagagcctg
cgcaatcagcttcaagccttggctaagagcctgcagaccaaggccaacaaataaatgaaagaaccagtgtttttccttgcttctcacttgcagctgctac
ttttgaccataagattgcgccctgaggtggagcaggacattaaggatccaacatgcctaaacatcttgcctttccctgtttgtatctgaggtacatcacc
cagtgattatactgctcacctaggcaagctcattaatcagatctctgacattaatgacaagcacaaattagaggtgaacgatgtaaagccaaagggaact
tcagtattaaaggcacatttaacctaagggcaagccttaaattatagcactaaactatccccagcatattctcctgcactaaacatgccttgtgtcctac
agatctccagctgactgccctgcctgaacaagaacctacataccttaggcaagttaagattcacagaaaagctaagacagaanagtatatgagcctctac
atgttatattttccatttggaacacaggcc
3' EST: CX995981 [+]ggattttatttttttttatttggataaaaattgcattgaaatagacatatattttacagcagagaggcactttataaagtaggtaaggcagcacatacag
cagaaatctagtgaaggcacaacgttattttaggtttaaaaaaggggagtgtattaagaaacataaagacaccagcaatgcccataatggtgccctacat
tttcaagagctggtgatacaatatggatatttagtaggttgagatacagacatccatgaaccctcttgtttaagaccccagctgtttggtacaagtgatt
ccttgcatggcctagtttactgcttgcccttggggcctgtgttccaaatggaaaatataacatgtagaggctcatatacttttctgtcttagcttttctg
tgaatcttaacttgcctaaggtatgtaggttcttgttcaggcagggcagtcagctggagatctgtaggacacaaggcatgtttagtgcaggagaatatgc
tggggatagtttagtgctataatttaaggcttgcccttaggttaaatgtgcctttaatactgaagttccctttggctttacatcgttcacctctaatttg
tgcttgtcattaatgtcagagatctgattaatgagcttgcctaggtgagcagtataatcactgggtgatgtacctcagatacaaacagggaaaggcaaga
tgtttaggcatgttggatccttaatgtcctgctccacctcanggcgcaatcttatggtcaaaagtagcagctgcaagtgagaagcaaggaaaaacactgg
ttctttcatttatttgttggccttggtctgcaggctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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