XB-CLONE-3535618
Clone Name: IMAGE:7720981
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Gene:
g3bp2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX996002 [+]
catccgacgggtatcgggactccttattccgcgtccgggggaatagtggcctcctgaccggctctggtagagcacattactttctgtaccaaagaaatgg
tgatggagaagccaagtcccctgcttgtagggcgggagtttgtgagacagtactacacacttttaaataaagcaccagactttttgcacaggttttatgg
aagaaattcttcttacgtacatggagggctggatgccactggaaagccacaagaagccgtttatggccaggctgaaattcacaaaaaggtgatgtctctt
cagttcagtgaatgtcgtaccaaaatccggcacgtagatgcacatgctacactgagcgatggcgttgtggtgcaagttatgggtgaactttccaataatg
gacagccaatgagaaagttcatgcagacgtttgtccttgctccagaaggttcggttccaaacaaattctatgtccataatgacatctttcgctatgaaga
tgaagtttttggtgactcagaagcagagatggatgaagaatctgaagaggaagtagaggaggagcaagaggaaaggcaaccttctcctgaaccagcgcaa
gagaacaccaacaatgcatactatgagcctcaccctgtttccaatggagtggaggagcctttagaggagccagttaatgaaccagaaccagaacctgaac
cagaaataaagaatgaggagataaagccagagacagaagaaaaagtgcaagaggagttggaagaaaaatcaccaaccccaccgcctgctgagcctgttgt
tctgccacaagaaccacccaaggcaagagtggatgttaaaccagaagctccagctcagtctctccgtgt
3' EST: CX996001 [+]aggtaccactgtaggctttactggatgttaattgtgactagattcagcggcgctggccagagaaacgaccctctacaggtggtgctcctccacgtccgct
gccaagcttttggaccattcctcctcgtggtggagggccccttccatccctgtcacgaaccattccaccactgatgagcccacgaggccctccaggtccc
ctatcattgcgtcgtatatctctgcggtcgtctccaccacgggtttccctttctcttgcagcccttgttttcttctcttccacatttaaccgtacctctc
cacgaaacataattggttttgaaagcaaaattcgctgaactggctcagagtcatcaaacacaacaaagccaaagtttggtagtttcccaccaacgccttt
ggtattaatacgaagctccatcacatttccataactcataaagaattctttcagttcactttcatcaatatcatgtggcaagtttccaacaaacagttgg
tgactgtcagggtagcggtacatacgacgattatcagactccgactgttcattgtcacttcgacctcctacccttggacctcttggctgaaaagctggtc
tttcccgaggacgctgttctcttacacggagagactgagctggagcttctggtttaacatccactcttgccttgggtggttcttgtggcagaacaacagg
ctcagcaggcggtggggttggtgatttttcttccaactcctcttgcactttttcttctgtctctggctttatctcctcattctttatttctggttcaggt
tctggttctggttcattaactggctcctctaaaggctcctccactccattgnaaacagggtgaggctcatagtatgcattgttggtgttctcttgcgctg
gttc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: