XB-CLONE-3535612
Clone Name: IMAGE:7721077
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Gene:
arrdc2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX996006 [+]
gtgccattggtgattggtactatcccactgcacccatttggaagtaggacctccagtgttggaagtcagtatagtgtcaacttggaatggctgcgtatga
ctgtacctgaacagcctgaacctcctcctgattattcttcagtggtgtctgaagaggaggcagaacacaacctttctcctccacatcattctagcatggg
ctgtttgctagaagggccattctatgcttacatccaagaattcagaaacagacctcctcctctttactccgaggttgatcctaatcctcccactgcagac
atcagaccacgttgcatgacctgctgaaagatgactgccagcaaactgaattgtctatgtaacgtaaagcagttgagcttagactgaaaatgtcctcacc
tccaactggaggtttcctctgggagaactgatccagaggtaggaggaaacagacttatgcctgtcttcccatggagaaatgaagctttccatgcaagaga
tgggtctggtgttccagtgcatcaccagtgtctcccagaaagcagtctgtaaaaggaagaggaggcgaggaatgatcattttaaccactattgaaccttt
gagtcagtgattatagtctgcttataatgtgattataatctgtttattgagctctggggaaaaacaggctttctgcacttctaaagaaaaaactaagctg
ttatgagatgttactaaaactgtaatagtacagcatttttatttttttctagacaattgtacattctagctgaaattgtcaaatgcctgacatcacccag
catgttgcaggctttgcttgcccagagcacagaaaccaaactgtntagggctaagcatt
3' EST: CX996005 [+]ttcaatgcagccaaccaatcgcaaatgcagtaaaaatatttacagtgtaaatgcaaagaaccataaataccacattatatcttctgcaattcacagatct
gctgtgagaataagatatctccagcaataaagaagttgcagcagtaaggttcacaggtctcctgattttgttgtaaatttaaagcaagtgtgcagccctt
acacaaacagcaactggaatgttcactttgttttccgcagttttttgttttgaattatacagtgatcttaaggcaacaaaatctgaagtttttttttttt
gtttgttttacagggaatacagggaatatctggaaatttcccactgaagtattttttcaacagcccgagggtcatgagtaatcccatctcagagtttaaa
gtcctgacttatatgtaaggtcacttaatagaatgaaaaggcactgtagcaccaaattccgctttcaaagcaatcctctttcattaatactgaaaaaata
taaggtgattttacagattgaataaataataaatcaatgtgcttgaaatataaacttacaaagcttacactattacaattttataaagttcactttcaac
tcataattaatatgtatcttttttttttctcccttaagaggacttaaaaaaaaaaaaaaaaaaggtttgctttaaggctactgctgccatggcatggaat
aacaaattaaggcagatactgtgtgtatagctattccataggtcaagcagatgtcataataaaagtaaacttttttcttttttagatcccagttcaatct
ttggttgtatgcaggcactgtagaaattacaggggg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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