XB-CLONE-3535548
Clone Name: IMAGE:7721131
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Gene:
tmem14a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX996050 [+]
taagcaggccatacactgaaagatctgcttgtttagtgaggttgccaaatgagcagatctttccctaatatgcccaacttaaatgggaagatattgcatt
gatccaattgtttggctgtcggcaagatgtaggctgtcggggcgaggaccgcatcaacgagcccatacactccttgccctaacgggattttgatacctac
ccaaatctggctggttttgaaccagagaattacattggggatgtttcacaatgctctacttttcttctttgcacagtgacagctgtgcctcacactacct
cttaccaacttatcaatggttttgggaagtcattcttccttttcttcagtctccatttttcttctcaggcacatgaggctctttaaggagtttatgtggc
gcgaaacattgtgctaaattgcacaaaaccaacccctgtagactagccctaaactttttgggcagctattatctgataagttttgtgccagcactactta
aaaacatagagcttaaagcttccaagtgccccatttagaatttccctaaactgtccagtccctagggcacaaacttattctgctttcttcaactaatttc
tagtgttgtttagtctgctgttagtgaaacattacacctctgtgttgagtgctgtttcttgtgtgaggaatctgaaataacaggcatggtctcaatctta
gaggtgaagatacagagtaacanagttaatgtgtgttcagaagtcttggaactttgttttataaagcaggttgtgaaatcaaaaaaataaccataattca
caataatattatttc
3' EST: CX996049 [+]tgtattttatttaatgttgtacaaattgttgctgaaaagcatcatgaattgtacaaaatagtaaaaaaaaaatattacaaaaaggaacagttttctaaaa
aagaatttgcaagttttgattactaaatgatcccataaagctgattaaatgcttaggagaagtgcatacaactgtaagagtaaattgtgtagaacactga
acactgcaataaaattagcagtaaaattaagacaaaatttcaggtagcaacagtaataacaacaaagatgttttttatttaacactctgccctatgccat
gtgttgccatgtttgtaaacaaacatggaacagatcaaacagtcatgggaaaatgaagaagttagctaattaagtaagcattgtagtccttttaatgcag
gtgccatagactctacgttctacgagaaaaaaaaatggtaagtaatgtcaatacagtggtactcagatacaaaagataaaaggtaaaagttgtaaggcaa
acaataatctgttacattctaccactgggccagtactcctcttcctcagacaggtcgtgccaccatatttttccactttttgaggcattccctgtgccac
cctaagtaagcacacccacagtgcaaaaattcttccccaacatctatactgcagacacacccagcatcggtaagggctacaattctactaaatactgtat
agcagggctatgttcaaaggaaaagtaccttgggccagtgcatttgaagaggagcgctagcccagggtaagaattaagcaacaagcttaacaactggtta
aaccacagtgatcctacctttttttctaggaatttctctctatctctgtctaataagggagcagtgcagatacatactggcctggatttgtgaggaggcc
acaaaggcccagtctagggcagcacaaatttaggggttgcatgcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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