XB-CLONE-3535545
Clone Name: IMAGE:7721179
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Gene:
cs
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX996052 [+]
ccagagagaatttgcacttaaacatcttccaaatgaccctatgttcaaactggtggctcagctttacaagattgttcccaatgtactgttggaacaaggc
aaagccaagaatccatggcctaatgtggatgctcatagtggggtcctcctgcagtattacggcatgaaggagatgaactactacacagttctctttggcg
tatcgcgggcacttggtgttctggctcagcttatctggagccgagctcttggattccccctggaaagaccaaaatcaatgagcacagatggcctcatgca
gctggtcggatcaaagtctggctaatcaaaacgggaatcgccagggcccctccattttgtatagagggctctttaaaaaaagacagtacaccttttgttt
caacggactgctttgcaccccactgttttctgaattccgatgagaaaacatgtctggttatttatgctgaaaatccatcttgcagctcagtctcagcccc
ttttcttgctgaagagatctgtattcagttctccatcaggaaatgggatcttgtgctgctggttagaatcagaactctggaaccccctcagtcacaatct
gttaactcacagaccccatcattctgcttatttataattcagccacgggcagtcagtgcctcctaattttgttactaatctgcagaagcagtggcccata
ggttttcatacacaggcttgtatggcacagcagccccctttatatgtcccattgtgaaacgcanaacctgctatagacttgcctgtgcttagcacaacac
tgcgcctggcttcatatcttcaggagcatgggtttcattctcttactgggaaatgtgattatgtttctgcaaccatttaactgctgtaggttggct
3' EST: CX996051 [+]ttttgtaggtaataaaaacatttattttgcatttatacagaacaaccttaagtctgttggtaagttgggatatcaatccataatttgcagagcatttgat
ggccaattagtctgcactgacaagaacaggttaggcaataccacatgggaaaaggctgacaggtggacaggagtgtattaacaaatgggtcacaggatcc
ctttttgggtctctaggtggacagtcatgctgtagagcagagatgccaaactgtaacccccaacagctgggaattatgagtcctaatatcactaactaga
ctttgcaaaagggctggcagcctgacattcctgcacaagaggaagacaaattaagcagcttgggaaagtgcacttgcagatctctgaggattaatgaact
agtgcagaaggagtgggaaatgcacactaacaagcacaccgacactgtataatgagcatttattcagtccaactgtggcctttggatagcagaaccctgt
aatcaaacaagaccccatagggtagcaatgcttaaggctcccaggcttttgtagtccttgggatgatgggagctgatcactgcggcaagaattgctagac
tgctgccacaagcaaggaaggggctaaagtaaaggtagctttaaaaaaaaaaacaaaaaaaaaaaaaaacaaaccagtagaaactctctctaactgaatc
ctttgaattcacagttgtgtttgtaaagcactaaaaggtcgttagcaatattttaaggcccagtacagcacaggaggatccagagtgctggaacacataa
aaagagctgcaaataaaagcataacgacagtagagccttactttagtagggggacattaaagcagcacccatccacataattcttgctcatggacatttc
ta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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