XB-CLONE-3535539
Clone Name: IMAGE:7721323
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Gene:
cntrl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX996056 [+]
ctctcttggaagatttaagtgctcaaaaaggagagctcaatgcccaagtgagcgagaagaaggcacagctggggcgactgaaacaggaaatccttcaaga
agaagataatctccaaacattggcttcccagataaataagctcaaatctgagctgaaacacgtgttggagatggtgcaactggagaacaacgaactgcag
ggcttgaagctgcagcacaaccagaaggtggatgagatggagaacatacagacaacccttctagaggggaagttagaattggaaaacctgcagcggacct
ctcaacgcctaaacggggaggtggagcggcagagacaagtgctggagaaagaccatagggaaatggagcttctgatgacgcagatgcacaccctccagga
gaatgtggcttccctgggcagcgacaaagcccgtgtggaggagagcagccagcagctggagaagaagctcgcccaggccacaaaaacactcgcagctacg
gaggagaataaccgagcggccagtagcaatgcagagaagttggcgtctgaaatcaaacggttacagaaagagatcgaccagctccacaaacagaagcatt
ctctgaggcaggaagggtcggtggtacagcagcaagtgcaagatagaacagatgagttgaacttacttaaggatgagcttaacgataccagggagcaact
gaagctaattgagcaggacctgagaaatgccacaagggctcgtgacgagatgctcagggaaaagaccaccctacaggcggccattgaggagagttcggcg
aggtacaaatctctgcaggacaaagaggccccggaagaacagcagctg
3' EST: CX996055 [+]ggcgagaggccggagacacaataatacaaaatagaaatgtataaaaataaattagaagaagtagttagtgcagggtcagacttgccggtgcgtctgggaa
atggatgcagtgtcctatgggaacagccgacacgtctgggaaatcacaggtcattggttggtgcttgtccctggtcagtttaaggcagaatgtggtgcgt
atagagagcgctgaagtgctgagtgcctcccccggccgtgtctgtaacgtgggagatatggattccctgacagggaggactgtgggaaagttcatggagg
gaggagttgaccccggagaggttctgggagtgcagccaagactcggccgagctgttactgaccagttcatccagcgcctccacttggcgcctcaggctgt
gcagggtcccctccgtgtggcgtttggcgcggctgagggcgtcggcttggctggacatgcgttggcgcagctgagccttcagtctgtcttcgtgttgctg
cagtttctcgcgcagggattccccgtgccaatgttcctccatcatgtgatctttgcccctcccagctctgtccgtatgtggcggctgtgacgctgtttca
ttggccgagaagggaaagttctcctttaggctttctaggcgagaatgcaggctgcggtttgactccgcctccacgtcccccaagagctcgcgctcctcca
cgcagcgcagatctcgcaggccgctttcgatttcgtttttaacctgagtctttaacgctctcatcgcttccctcacttcctgctgccgcttgtccacttc
ctcttgggttttgagctggttg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: