XB-CLONE-3535500
Clone Name: IMAGE:7721183
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Gene:
atp6v0d1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX996082 [+]
agaatgcattcagtgaaataggatttatnctttaaatatcgtttttaacattgcacatataaggataatatggtgatcttcttttccagaataacagtta
tattctttaacactgaaaatcagaaaatataaaaataccactcaatgccacaattctgaaaattaaccaaaatgacaagaagggcaatggatttccacat
caaacgaaatgttcttgtcttcacatcatacaggggaaacactctcatgtgattaattgggactatgctggaatctactgtatactgtatgcatgaagca
ttacatcattgcttaatgacagttataatgcgcaaaataaaattaaaacatacgatttaggtttcttttaatgggtgctgataagcgaatcattccctta
aaatgcgctttttttttcttaaaccttagaattatgtaaaacctgctgtccctataatgacccaaaagggtaccaaaaaagtaaagcagtgctgtagtgc
cattttacctgtcccagtcattcgctggcagactcaaattcagatatgtgcagtaaaatgcagttttttactctactgcacaaacgctcatgtctgaaaa
gcaagatggggcacaagctgtattacatctttccctggttttgcatgttttttccctaacaggagcaccagcgcagagataaagtttgtgatcagatcgc
atagatgttacatttaggcatagtgattaaaggtttatacaccatactgtcatatggtaaagattaatgatgcaacttctacgatgtaacatctctaagt
gagctngtgcaccataaataaaaaaaaaaaacagtagggtttttatgggtgcatttcatgaaaat
3' EST: CX996081 [+]gtagttacctcaaagaaccacaggagattttatttaatatgtgcaatgccagtggccctttttccttcttcacatgaggtgtgatgcatgttatgcatat
atgacaaatatactatggttagattcaaattctttaatataaggggtatacgtaaattgagacttatctgttaaggctgatgcacacagacaattttctc
tttaccctctgtttttagattagggtagaggcagtaagtacaagcagactgctagtgcaagcacatacaggtcaacagacattgtcatgtgtttttgcca
tgccagtgcttttgtcacaggtcacaggtcttgcatcttggcaggagataatgtgcaagctggcactgagaaagcttctatttactcaaggtcttacaag
gaaattaatttgtggccaacccattaactgaattttacaacagaatgtataatgatttttaacttaaaggaacagtaagggtgaagacacatggagctac
ttagtagcagctaacaccaggaaataaaaatgttttaaagtaaagtgcgctggtaagggttgggtgttttcttcggaaacggtactgtagtttgcgtaga
cggggtttctgggtagccatggaggcagccattcaagcttgaaaaaaaggagaaaaggcacaggccacatacagataagttctatataacataattggag
tctacacataacatagtgtaacatagttatatatacatctgatgtataacttcagccttttttcaattgaatggctgcccccaggctacacagcagcttt
gtttgtgtggactatagtattgtttctgaagcaagcacacaacttttaccagtgcagggcaatagtgcat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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