XB-CLONE-3535485
Clone Name: IMAGE:7721089
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Gene:
MGC75753
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX996092 [+]
gcgcgaggtgaacatcggacggagtgagagggctagctctggcctgcttcccccccttcagcgaaacactcgggggtagaaagttgaggactctggctgc
cggagatcacaaaggaatcgcgtacgattctgtcctgcctatacgatcgaagagagagacccgctgctgttgatgtttatatagctgtgaggattcaacc
ttatatgccacaatgatgactgagatctcaacagctgctgcgtttatcacaagtttgctgaaaacaaccggggtcatagaggagaggcagctgcagcact
tctgccaggtcctacaggaggccatgaaggatcattataaacaccactggtttccgcagaaccctttcaaaggatcagggtaccgttgcttgagaattaa
ccacaaaatggaccccttgataggacaggcagcaggtcacattgggctcagccaccagagactgttccagcttctccccagcgagctgactctttgggtt
gacccttttgaagtctcctatcgcattggtgaggatgggtccatctgtgtattgtatgaaggagccgcagcagggggcaagcctattgaaaaccatgcaa
gctgcaaggatgcacttagaattggccggaatagcccctccaaaaattacagcataatgacagtttctagttaaacagctganaaataaatcaggaattg
tacaattacgtatgtgtccctcttctgccttgatttgcgttaatgtttcatcttcattccataaacattaaaactgtaatactgccaaaaatgtaccgtc
ttgttgcagaaggtggatgtagaataccatctggggcaaaaagctc
3' EST: CX996091 [+]aaaaagagccaatggttataccaaaatataaattctctcttgactcatgatttcattttacaatgtacaatttcagagaccggagcgagaaaatgtcata
aaagaaagacagaaatgacaataagagctttttgccccagatggtattctacattcaccttctgcaacaagacggtacatttttggcagtattacagttt
taatgtttatggaatgaagatgaaacattaacgcaaatcaaggcagaagagggacacatacgtaattgtacaattcctgatttatttttcagctgtttaa
ctagaaactgtcattatgctgtaatttttggaggggctattccggccaattctaagtgcatccttgcagcttgcatggttttcaataggcttgccccctg
ctgcggctccttcatacaatacacagatggacccatcctcaccaatgcgataggagacttcaaaagggtcaacccaaagagtcagctcgctggggagaag
ctggaacagtctctggtggctgagcccaatgtgacctgctgcctgtcctatcaaggggtccattttgtggttaattctcaagcaacggtaccctgatcct
ttgaaagggttctgcggaaaccagtggtgtttataatgatccttcatggcctcctgtaggacctggcagaagtgctgcagctgcctctcctctatgaccc
cggttgttttcagcaaacttgtgataaacgcagcagctgttgagatctcagtcatcattgtggcatataaggttgaatcctcacagctatataaacatca
acagcagcgggtctctctcttcgatcgtataggcaggacagaatcgtacgcg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: