XB-CLONE-3535469
Clone Name: IMAGE:7721091
|
Gene:
alkbh5
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX996104 [+]
ccgcctgggtggagcacagggtcatccccgagggcttcgtcaacagcgcggtcatcaacgactaccagcccggcggctgcatcgtgtcgcacgtcgatcc
catacacatcttcgagcgaccgatcgtgtccgtgtccttcttcagcgactcggcgctctgcttcggctgcaagtttcagttcaagcccatcagggtgtcc
gaaccggtcttctttctgcccgtacagaggggcagcgtcactgtactcagcggctacgcggctgatgaaatcacacactgcatccgaccacaggacatca
aggaaaggcgcgccgtcgtcatacttaggaaaaccagaacagaggctcctcgactggaaatgaaatcactgagcagcagttaccaacctgagcgcctgca
aggcagcaacaggcagcatatactaaaaccaaagcggtcccatcgcaaggctgacccagatgctgctcacagacctcgcatcctcgaaatggataaagag
gagaacagaaggtcggtacttttaccaaaacagaggaggcggagccacttcagctccgagaactattggcgaaggtcacatgattatgtggacacctata
cagaaacgggagaagacgacggcagcccagtccgcaaagttaaaatgagacgacactgaggcggccacttttctgatcccaagaagttctgactttttca
gactttctgattttttgtttctcccactcggctctccaccgtttctctgatattgccccgtggctatgggggtgcattgtaaatgatggttccccgctgc
tcgtgtttataccaccccacccgggtctgattgtcctcatcacancac
3' EST: CX996103 [+]gcagggagtgctttttcatataaagaacttgtagtttccctttaaaggatgcagaagagagtgagtgacttggccagctgtattctcaagtcagcaatcc
ataaaccccctggcaccttgtcacaaccatacagcatcttccagctggggggtgagggggtgttaaacacacagaaatctgcctaagcagaactgcatca
ataattatgggttgttcccctgattagtgattagaatggtttcttttatttagggggagggggaaacagaagaaaggggtgagggttggagaaactgtgc
taaacaaaacattgaaacatcaaccgactgttccacgggagtctttgtatattgtactccgacgcgctctggtccctcattgcagctgccaagcaagcaa
tggggatccttactatacgccaaggtgcagcttttaactcacagcgctgacagacttcctggcagccaccgattccaacagtattcagggtaacttgtgt
cccgctccaacagtattcatggtaccccttggcttttgcacacctccaatttttgttgtgtgatgaggacaatcagacccgggtggggtggtataaacac
gagcagcggggaaccatcatttacaatgcacccccatagccacggggcaatatcagagaaacggtggagagccgagtgggagaaacaaaaaatcagaaag
tctgaaaaagtcagaacttcttgggatcagaaaagtggccgcctcagtgtcgtctcattttaactttgcggactgggctgccgtcgtcttctcccgtttc
tgtataggtgtccacataatcatgtgac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: