XB-CLONE-3535396
Clone Name: IMAGE:7721193
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Gene:
sipa1l1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX996155 [+]
gagaaaatgcacagttgcgtcaagttccctcgcagttgcagagtccagtttcttcacgagtgtcatctgtaaaaggagatggcaaaatgcaaacacaaga
gcgtacagcaaatattccacgcagcatctccagtgatgggcgtcctttggagaataagaggctttctccaggttcagagatctacgtcacagtttcctct
gttgctattaaccgacctcagccgtgccgcaactctcccagtaacctttcctcatctagtgacacagggtctgcaagtggctctcaccgtagacaaaaat
caatgcctgaagggtttggagttggtcgtagatcacctgcttcacttgatcgacaaagcactcaaactgatacagcaagcagcggcaagtcaactcctag
ttggcagagaagtgaggatggagttcctgaacaaatcgaaccaacttgtcatctccctgctgtatctaaaatacatccatcatacagagacagcccaaca
ggaagactcattcgacaggaccctgttcttcatctctctccccacaagcagggacagacggatagccattactctagccattccagcacaaatactcttt
ccagtaacgcctcaagtgcacacagtgatgagaaatggtacgacagcggagaacggttagattctgacatgaacagttatacctacattcaaggcacatc
tgctgacagcggaatagacaccaccttatatggcaccaaccacgttaacgctgcctctcacggcaactcttcaacatctccccgctctggtccgccaaag
gaaaaagtggcccctctgtggcatagtgtcagtgatgtgggattaatcactgacaggacttgtgacagagactctcatgtattagatgttaaagcggga
3' EST: CX996154 [+]tttctctggagcctctggtaaaagaatttgctgtaaaattctaataaatagcccacatttaactactccctgttcaaaataggaactcagagcaagacat
tagagtactctagagagagccttgcaaaagattcaatttacaaattatatcaagcattagaatataaatctccttcatacagttattaaaaaaagacact
aaaacactccttttttctgtgttttatcgcatttctacaaataatcccagaagggggaacaatcatcagactgcaagcttttcaaatctttgtgtcaggg
aaataggttatttctgtacatgccactcagctattagaaacatttcaaacattgtaaaggaaagaatccaatttattgaatttttttcagtgtcacaaac
ggtactgataaagcataacacgctgaaaattttcttacaaaatataaatatctctttgcctacatagaatctcatagcaaatatagggacagttctcaaa
attgtaatttagacagatttcaaactgtttttgtgtgttttgatcttgtccagctgtaaaataaaaatgcgcgtgggcaaacaaatgctgtatattagaa
gagacaagttgtgccagccaagccttcggatccgagacactcaccgagaactgtacttcccaaaatctgtagggatgtgtttgttgtagtatataaggtt
tataaagcacttaaataatgccttttgaaatagttatacatgtaattgtttcactatatatctagtaactactagacccaaagttattcaccttcttttg
caatatattaaaaacaatgcccacttcaggctatgtagcaacatattatccccttanaatcatggtatgcagaaagtatatcaaattactaaaatacatt
tgtacactatt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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