XB-CLONE-3535384
Clone Name: IMAGE:7721003
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Gene:
mrtfa
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX996163 [+]
cttaaacagacatctcccagtcacccagctgtacaaacggtgccacaggttgaaatgccaccaccgccttcacctattcagtctgcactaggaaggctgg
aggatttcctagagagttcaactggcagcccactactacgggggaaccaagatggtcctgcttttatgcctcttattgatgacctccatagccagatgct
gagcagcttggccatcttagatcacccaccctcacctatggacacatctgatctgcacttctcccctgtaggagactccctaggattagacatttctgaa
cctcctttggatggtatggactggcttgagctgtctgagcctccggctatgaatctgactccactcagcacagctacccccagtgtcttttccactgatt
tcttggacagccatgatctgcagttacactgggattcctgtttataacctagctattagttggggattaagaaacaaatgtggatgaagaacgagttcag
cacttttcctggcccctttaggaaattccttctttgccccccttgagtggtacttacggaaactgaaagaaaatgctcagttcagggtgccaaattgtag
ggaatccttagttgctacccttgagaaggcccctgactaccttcattaaagacacatgtaagatgggtaagcaagtcaataagaactatgcattgtctct
aaccagttgttcatccttgatgcactgttcaaatgtgccacaagcttccttagcttcagataccttgccctttatattgctttctgtgtcanagctcatc
ccaagcacttgtaagaaactgtccagtgctgtaagtcacccctggtcgatatgcctgtctggaaggtagtgatgttatcttgccatttgtaactactggg
tagaaatacatggctggggattcagcatctgatatc
3' EST: CX996162 [+]tttttcaccttccacagctctttattccatgatgtgatttatttttttttaaatttttttggtattttacttccttttatgaatttactcattttttttg
cctatgtctcacaacattactgagaaaagacagatattcatccgcattttacattcatttcacagatgtgagcaaggattccaggtacccagcattcatg
atgggaaaacataaaaaagaaaggaaagagaaaaataaattgttcaccaaaccagaggtgggtcgatagtgaaagaacccaaaccgtttgcagaacacac
caaggatataaactgtcatgagttcggcaagggttccagttgggtgaacagaagtgccatagattgaacacgatatgagcaactataaggggaaaaagtg
gggctttgcgtttacaccaagagtcatggctgaacttaatctcctcagcagtttcagattcctgggaagggggacctagaaattatacagcagcactaac
cttagtctttggatatcagatgctgaattcccagccatgtatttctacccagtagttacaaatggcaagataacatcactaccttccagacaggcatatc
gaccaggggtgacttacagcactggacagtttcttacaagtgcttgggatgagctttgacacagaaagcaatataaagggcaaggtatctgaagctaagg
aagcttgtggcacatttgaacagtgcatcaaggatgaacaactggttagagacaatgcatagttcttattgacttgcttacccatcttacatgtgtcttt
aatgaaggtagtcaggggccttcttcagggtagcaactaaagattccctacaat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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