XB-CLONE-3535280
Clone Name: IMAGE:7721061
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Gene:
eef2.2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX996237 [+]
cttgaagcgcctggctaaatctgatcctatggttcagtgtattattgaagagtcaggagaacatatcattgccggtgctggagagctgcatctagaaatc
tgccttaaagaccttgaagaggaccatgcgtgtattcctctcaagaaatccgaccccgtggtttcctaccgcgagaccgtaagcgaggaatccagccagc
tctgcttgtccaaatctccaaacaagcacaatcgtttattcatgaaggcgagacccttcccagacggactggccgaagacattgacaagggggacgtaag
ctccaggcaggaattaaagcaacgcgcccgctacttggcagagaagtacgaatgggacgtggcagaggcccgaaagatttggtgctttggccctgatgga
acaggacccaacatcttagtggatgttaccaaaggcgtccagtatctaaatgagatcaaggacagcgttgtggctggattccagtgggccaccaaagagg
gtgtgctgtgtgaggagaacttgagaggagccaggtttgacattcatgacgtcacactgcatgctgatgccatccatcgaggaggcgggcaaataatccc
cacggccaggagagtcctgtatgcctccgccctcacagctcagccccgcctcatggaacccatctatctagtagagattcagtgcccagagcaggtagta
ggaggcatctatggcgtactgaacaggaagcgcggccatgtctttgaagaatcccaagtagcagggacaccaatgtttgtggtgaaggcgtatcttcccc
gtaatgaatcatttggttttactgctgatttacgctctaac
3' EST: CX996236 [+]catattaatgtcatttgtttttagagggcaaaggatcctcctggtgttctcctagtcagaggtaagtgctgggattctgctgtgtgatgagattggaatc
tggttcatgagaaaaagcggcgttgctgtaaatcacaagactgacaggacctagggtctgagaccggggaggggttacaatttatcgaggaagttatcca
ggggtgggatcccttccttcaagcctttccgcttgcgggtttctccaacaacttggctggggcgggaggtgctgtcaaatggatctccgggcaggatctg
ccagtgatcgaacacgcactgggggaaagcctggccgccggtgttagagcgtaaatcagcagtaaaaccaaatgattcatttacgggaagatacgccttc
accacaaacattggtgtccctgctacttgggattcttcaaagacatggccgcgcttcctgttcagtacgccatagatgcctcctactacctgctctgggc
actgaatctctactagatagatgggttccatgaggcggggctgagctgtgagggcggaggcatacaggactctcctggccgtggggattatttgcccgcc
tcctcgatggatggcatcagcatgcagtgtgacgtcatgaatgtcaaacctggctcctctcaagttctcctcacacagcacaccctctttggtggcccac
tggaatccagccacaacgctgtccttgatctcatttagatactggacgcctttggtaacatccactaagatgttgggtcctgttccatcagggccaaagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: