XB-CLONE-3535271
Clone Name: IMAGE:7721205
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Gene:
c6h6orf62
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX996243 [+]
cgagattccagctattccctggaaagttcccttgagcttctacagaaggatatagtacagcttcatgcacctcgctaccagtccatgcgcagggatgtta
ttggatgcactcaagagatggactttattctttggccaaggaacgacatcgagaagattgtctgtcttcttttttcaagatggaagggatctgaagagcc
ctacaggccagttcaggccaagtttgaatttcatcatggcgattatgaaaaacatcttctgcacattctgagccggaaagacaagactggtattgtttta
aacaatcctagccagtcagtgtttctatttattgatagacaacatttgcagactcctaaaagcaaagccacaatcttcaagttatgcagcatctgtctct
atctgccacaggaacaactcactcattgggtggttggcactattgaggatcaccttcgcccttacatgccagaataaagttttgaccagccaaactggga
agatcagttattagcagcaatttattttcttctaaattacagaagaacatggactcatgctcagaacacttgtatgttgtgaatcttcatgctggatatt
ttttcctcattttgtgtcagaacttttttttttttatttttattttttttgctttttactttgttgtgtgtggaccttgtgaaaagagaggatgtttaat
acatctttgttatagattctcaaaatgtgaattgatgggactgtggcacactgaatcaaatgtatctgaaatgacaaggtattttgtttagtggtcttcc
catttgcactggtcattcccctaacatcatttagatgacagtattg
3' EST: CX996242 [+]cagatttcaactcatttattttaatttccaatgtaatccaatttcaaacaaaagctagatacaattttaaaccaggtaagcactagtattagccaaaggg
aataggcctatacaagttgattcataaaaggtactgaaaccttttagaaaagccttctcttaaacaatacagtagtcaaccttatcattgcacaaaataa
aatgattctttgaaccaaatttcacacttcaaacttcttttacaatttgtaacggtgttctgacattactttattgaaaccaccaagggtttttcttttt
tctatagaaatattacttcattttatgaaaaagtaatatttgcacaaatcaccagcaggtttctatttcagttcctatcttcagaaacatggtttctgag
gtgtcaacgaattttaagtcgttttcatatagttactgcagactcctagacttatgtcagtacccacaggtaggggaactctgttatgcagcaactggga
gaatataccaaagtgtaaaagtgccattactcgttcactgccctgcaagctgaactacgatatgaaagcaaatgctgaataaagtttctttttcaataca
tctgagccagtcttcattgtaagccaacatagggtatgctttaagagattcagatagtgctttcataatacagtgaagcagcaaagctatttatacaact
tacattcattttttttcaccctcaatactgtcatctaaatgatgttaggggaatgaccagtgcaaatgggaagaccactaaacaaaataccttgtcattt
cagatacatttgattcagtgtgccacagtcccatcaattcacattttgagaatctataacaaagatgtattaaacatcctct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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