XB-CLONE-3535217
Clone Name: IMAGE:7721068
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Gene:
gnl2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX996284 [+]
ctttgctggccttgggagtctgttttctttttgttgcgattcttgtttttgacattatgagtttcataataccgcacaccaactttcttagagtgctgct
ggcgctcttgccttctcctctcttttgatgaaagtttcttttgtggctgttcactatcttcttcatcggcatcacattgttttcttttctttccttttcc
tttatcttgttggtcctcacttggttttgtaaacaggttttctgtcagatgcttaggaattcttaaagcagaaaatcttttggctttggctttgtctagg
aattttttgtactttgcaatccgatcatctaactcttttaccagtgctttagaacttctgtctttggtgcttgaatcctttgtttcacccattagcatat
cactgctttcctcttctgtttcttcttctgcagagtcctcttcttcagactcaacatcagagatatctgggattccttcaataggtactaaatcttcctc
tgagaattctggtgccacgctgatcttgccaaagttttgtcgtacatgggacatgagatctttgatctgatttctgtctggattttcttgagaagcttct
gttgtatcattttcctctgactgctccttagtaacactactggtatctgcaggagtagccaatgcctgtgctgtctcggaattatgttcagcattaggag
gtttaacatagaacggaatcctccccctctgccagtcattaagaaccatttttgagacagtttgacggtctggttcaccgcccttt
3' EST: CX996283 [+]ctttagccggttgaagtcaccgcagtaaaaatggtgaagccgcgttttaagggacggagcacgatcaacccttctacctccagcagcaatcctgatcgtg
tcaagggtgctggaggtaacaacatgagagaccgggcaacaatcaagcggcttaatatgtaccggcagaaagagcggcggaacaaccgtggcaaagtcat
taaaaccctgcagcaccaatcatcagttgcccccggaactgttgccagggtggaacccaatatcaaatggtttgggaacacccgtgttgttaagcagtcg
tctttgcaaaaattccaggacgaaatggatgctgtaatgaaagatccatacagagtggtgatgaaacagagcaagctgcctatgtctcttctgcatgatc
gtgtaaaacctcataattccaaagttcacattcttgacactgaaacatttgagacaacctttggccccaaagctcaacgaaagaggccaaacctttcagt
ctgtgatgtgcaagagctaatggagaatgcagaatccttggcagaaaattataaccagcagaatgacagagatctggttaaagaggacactggtgtcaga
gatgaagcccgtgaggagatctataagaaaggccagtctaagcgaatctggggagaactctataaggtgattgattcatctgatgtgattgtgcaagtct
tggattccagagatcccatgggtactcgttcgccccatatcgaatcttatctcaagaaagaaaagccttggaagcatcttatttttgtcctaaacaagtg
tgacttggtacctgcatgggctacgaaacgttgggttgccacattatctcaggaatatccaacattagcttttcactccagccttaccaactcttttggg
aaaggag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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