XB-CLONE-3535208
Clone Name: IMAGE:7721212
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Gene:
clk3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX996290 [+]
agaggaggaggcgatccccaagcagagagcgagatggccgccgactacatacacacagaatatccccacgtgcacacagattttctccgtataggtcttg
cttaagaagctggaaaagggacacattctttgatcggtgcaagaggacgcactctagtgaaattcgtttttctcaagggcacagtacttcatcccacagc
aaaagctattcctactccacccggtctccagctcgcccacactcaagagaccgtacaaagacaaaagctgagaaacagcctcataagatctctcacaaac
acagcactaggtctggtagctactcttcctcgaagagccagcggagcactaaggacagccagaatgtggagaatgataaggaagggcacctggtgtgccg
gacgggagaacgcatccaggagagatatgagattgttggagacctaggaaaagggacctttggcaaagtggtggagtgtctggatcttgccagaggcaac
tcccgagtagctctcaagatcatccggaatgtgaggaaataccgggaggcagcgcagctggaaataaatgtcttggagaaaatcaaagagcgagatcagg
acaacaaaaatatgtgtgtcttgatgagggactggtttgatttccatggacatgtctgcattgcctttgagttgctggggaaaagcacttttgagttcca
gaaagagaataatttcttaccgtatcctctggcacatattcggcacatggccttccagttgtgccaagcattanagtttttgcatgagaatcaactgacg
catactgacctcaaactgagaacattctctttgtg
3' EST: CX996289 [+]caaactttcagtaacaaagacagggaaactattaagatcctccctataatataagtggcacaccagtaagagggaagtgtgctttgtaatattctggttt
agaccttgcacctccttccccactgaaattcagctgagtacttgctcagtgctctacctccctgagtgcacccatatatacatttgtatgaactattcac
ctctaataaatagaaaagcaacatttgtgttacatggctgcagcttcccacaatgctctgcactaaaactgacagtaggactcagacgatgaactataag
tatccaccatgatcacaggtacgcagagagatcacattgttcttaatggctcttgcaccataatcaagcaaattgacaaacgcttgaagtgtgcaacttc
tatccttgattgctgttgactaaattgtgcatgtgtgtgtagcactggtcctcttaaacaaagaaaacaatattacagatatattgggaaatatgttcag
agcaagaagcgtgccacagtgatgatacaatcagtccgaatgacagcaatatactcgctaaagtgtccagtcaaactctgttcgtgtcatctgctgaggt
ctcttgagattcggttacccagagcattctcttctggcttcaagcaagtgaaaaattgatgttccagggcttcctgaagcgtgatgcgcagcgcaggtct
gaactccagcatcctgctcagcaagtcaaacagctgcacgtgttccgcagaatcactgctcttgtaactcataagttgatgacagtttttgctgacatat
cgaccatctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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