XB-CLONE-3535181
Clone Name: IMAGE:7721310
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Gene:
slc25a32
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC157211 [+]
CTAGGTGGAGGTCACACACGCCCCCTTTCCCCGCATGATGTAACGTGTGTCTGTATGTGTCAGGCTGGGGAGCCGGGGTACGTACGTTGCACATGGCTGC
TCCCTCTGACCCAGCGCAGCGGCCAGCCCTTCCGGCCAGTCCCCTGAATGCTGTGGGGCAGGTGTTCAGGCATGTTCGTTATGAAAACCTGGTGGCCGGC
CTGAGCGGGGGAGTCATTTCCACTCTTGTGCTGCACCCCCTGGACTTGGTTAAGATCCGCTTCGCAGTTAGCGATGGCCTGGAGTTACGACCCAAATACA
GGGGCATCTTGCACTGTTTGTCCACAGTGTGGCAGCGAGAGGGGCTGCGGGGTCTGTACCAAGGTGTTACCCCTAACATGTGGGGAGCTGGAGCCTCTTG
GGGCTTGTACTTCTTCTTCTATAATGCTGTTAAAGCCTACAAGAAAGAAGGGCGAGCAGAGGACCTGAGTGCCATAGAGCACCTTTTATCTGCTGCTGGT
GCAGGAGCCTTAACACTATGCTTTACAAACCCAATATGGGTTACTAAGACCCGGCTGGTTCTGCAATATGATGCTGGGATTGACTCCACAAAGCGGCAGT
ACAGAGGAATGTTCCATGCACTTGGGAAGATTTACAGACACGAGGGAATACCTGGGCTTTACAAGGGGTTTATCCCAGGCCTGCTGGGAACCTCCCACGG
GGCTCTTCAATTTATGGCCTATGAGGAACTAAAGATGGATTACAACAAGCATCTAAACCGTCCAAGTGACACAAAACTGAGCACACTGGAATATATCACA
ATGGCAGCGCTCTCTAAAATATTTGCAGTCAGTGCTACATACCCTTACCAGGTGGTGCGTGCCAGGTTACAGGATCAGCACAACAGATATACGGGTGTCA
TAGATGTGATCCGCAGGACATGGAGGAAAGAAGGAGTTCATGGCTTTTATAAAGGGATCGTTCCTAACATTCTGCGAGTGACTCCCGCTTGCTGCATCAC
TTTTGTGGTGTATGAAAAAGTGTCGCACTTCCTACTTGGCTTCAGGAACCACAGAGACTGAAATAAATTTGTGATCGAATGGCAAAGGATTTTTCCCAAT
GCTGAAATACAGAATTAGGAGAATCATTCAACATGGACCGACTCCAGAATTCCCAATGGACTTTTGAGAAGGAAACGCATATCATCTGTTTGGGGATGGG
GTTTAAACATGCCGGAATGCAGCTTTCTCTTTTATTTATATATTTTTTGTGTTAAAAATGGTAAAATGTTTATAGAACTTTTTTAATTTTTGCAAGACTT
TAAGTGCCAGTGAAGGTTACTGGTTATTTTTATTTATCTCTGCTGGTATTTTTACTTTATACAGAACTTACAGATTATAATTAACTGTTGGCAAGTAATA
AAAACTTCTATTCTGCTGAAAAAAAAAAAAAAAAAA
5' EST: CX996308 [+]ctaggtggaggtcacacacgccccctttccccgcatgatgtaacgtgtgtctgtatgtgtcaggctggggagccggggtacgtacgttgcacatggctgc
tccctctgacccagcgcagcggccagcccttccggccagtcccctgaatgctgtggggcaggtgttcaggcatgttcgttatgaaaacctggtggccggc
ctgagcgggggagtcatttccactcttgtgctgcaccccctggacttggttaagatccgcttcgcagttagcgatggcctggagttacgacccaaataca
ggggcatcttgcactgtttgtccacagtgtggcagcgagaggggctgcggggtctgtaccaaggtgttacccctaacatgtggggagctggagcctcttg
gggcttgtacttcttcttctataatgctgttaaagcctacaagaaagaagggcgagcagaggacctgagtgccatagagcaccttttatctgctgctggt
gcaggagccttaacactatgctttacaaacccaatatgggttactaagacccggctggttctgcaatatgatgctgggattgactccacaaagcggcagt
acagaggaatgttccatgcacttgggaagatttacagacacgagggaatacctgggctttacaaggggtttatcccaggcctgctgggaacctcccacgg
ggctcttcaatttatggcctatgaggaactaaagatggattacaacaagcatctaaaccgtccaagtgac
3' EST: CX996307 [+]cagcagaatagaagtttttattacttgccaacagttaattataatctgtaagttctgtataaagtaaaaataccagcagagataaataaaaataaccagt
aaccttcactggcacttaaagtcttgcaaaaattaaaaaagttctataaacattttaccatttttaacacaaaaaatatataaataaaagagaaagctgc
attccggcatgtttaaaccccatccccaaacagatgatatgcgtttccttctcaaaagtccattgggaattctggagtcggtccatgttgaatgattctc
ctaattctgtatttcagcattgggaaaaatcctttgccattcgatcacaaatttatttcagtctctgtggttcctgaagccaagtaggaagtgcgacact
ttttcatacaccacaaaagtgatgcagcaagcgggagtcactcgcagaatgttaggaacgatccctttataaaagccatgaactccttctttcctccatg
tcctgcggatcacatctatgacacccgtatatctgttgtgctgatcctgtaacctggcacgcaccacctggtaagggtatgtagcactgactgcaaatat
tttagagagcgctgccattgtgatatattccagtgtgctcagttttgtgtcacttggacggtttagatgcttgttgtaatccatctttagttcctcatag
gccataaattgaagagccccgtgggaggttcccagcaggcctgggataaaccccttgtaaagcccaggtattccctcgtgtctgtaaatcttcccaagtg
catggaacattcctctgtactgccgcttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: