XB-CLONE-3535055
Clone Name: IMAGE:7721130
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Gene:
ywhae
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX996393 [+]
catcgattcgttttatttaggtttgtttatttaagtttaatgttaattccatgctgtgtttcagtaagaacaattacagattctgtatctgtggctccag
tcagatatccagtagtacaaattagcttcaagttacacatactgaacgaaagataggttgagcgagagaaagaagggcaggaggggagaagagagaaacg
aaaagaaaatttaaacacgcatgcaggctattcaacaccagctccaacgctaacctgctctgaggagggagagtaaaaaggaggcaagaatgaattaacc
cggattgttgacctgttaccagcaccagagttttcagccacatttttgcacagtttgaaaacacttacagcaaaaccactacgaggacattcggaagcaa
tctgcaacgtcaagctctaaccacatttgaaacaatattatgaaattcagttcaaacatttatataaaataaaaaaaagccaccacacaagttctacagt
gcgattgactctggtctgccctaaggaataacgtcgtttgcttgttctatgtgtttctaaaaggtaatagtgctggatggcatggagacaatttaatgct
gaaaagcccccctatgcagtcctgttagtgtcttaaagaacctaaaatggctggcagcagcagcaaaatccacagtcattcactcttgcctttcttaaac
tgtgtaaaaatgatacacccaactgggcaggaacctaatcctgagaaccagcgtaaaagtcaaattttggaggtcctcagagtgagacgggatttttatt
ttggagggaaggggcagttggttagagagggtctcttcttggctttaatgtctcactgattttcatcttccacatcctgcagtgcatctttattctgttc
t
3' EST: CX996392 [+]caaaatttcatcctcgcaccgtggctcttactgtgctacttttcccatggttataataactcaagatcagtaggtgttggctgccttgaagactacagtg
tgagggaccatctaggataaactctaaagtgctcagttatggttgaaagttcttctgagcagtaagtcttctattttagaaagtctttttgtataacgat
attaacataaaagagagggcttgcttaagctaatagtactttgttttaaaggttggcaaaagcagcatttgatgatgccattgcagaactggacacactg
agtgaagaaagttacaaagactccacacttatcatgcaattgttacgtgataatctgacactatggacttcagatatgcaaggtgatggtgaagaacaga
ataaagatgcactgcaggatgtggaagatgaaaatcagtgagacattaaagccaagaagagaccctctctaaccaactgccccttccctccaaaataaaa
atcccgtctcactctgaggacctccaaaatttgacttttacgctggttctcaggattaggttcctgcccagttgggtgtatcatttttacacagtttaag
aaaggcaagagtgaatgactgtggattttgctgctgctgccagccattttaggttctttaagacactaacaggactgcataggggggcttttcagcatta
aattgtctccatgccatccagcactattaccttttagaaacacatagaacaagcaaacgacgttattccttagggcagaccagagtcaatcgcactgtag
aacttgtgtggtggcttttttttattttatataaatgtttgaactgaatttcataaatat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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