XB-CLONE-3535015
Clone Name: IMAGE:7721038
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Gene:
rnpep
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX996419 [+]
cataagtgtgatttatttctttaagtgctgcaaacagggaagactgcacacaggtaagccatacattttctaaggaccacagaaaactcgtggtcaggtt
tatcatttcattacacagggttgaaaaaaagaccagagtccatcaagtttaactctttcaagtaaacccagcatagacaacctatacttaccaatctata
tactcacataaattatatacaaaaacattaatactaactgtagatattagtatcacaatagccttggatactatgcttgttcaagaactcatgcaggccc
cccttaaaaggcattaacagaatcggtcattacaaaattactaggaagggcattccacaacctcactgccctcaccgtgaaaaatcacctacgctgcttt
agctttaatgctcagagctcctacagaaataaatcacggcaatgaaataaggcggagataatcagcccctctccctgaacctaaatggaatgaatgcaca
caaaatgggagaacaattacagaacaggctgaaaaatctgcagcactgaccgcactgacagttttagcagctagaagtccagctagaagaagtgacctgt
gcacatacgattttgcaaagggcatgtcgtcatatatgtcgatatcaacgtgggggccccaaaaacactaacctgcctacagccttaatctagctctggt
cctatttgagaagactgtgcacaactggaagcagaattttagcatggcatttttgtataaaacattcaaaatggtcagctttgtgcagaggcttnaactt
tgtggcttanacatttagtcattcagtaataatataacatccatgtgccatggatcagagcaacaatataaggcagacagagtgatgtttattttggttt
attgcattttcagtctgaa
3' EST: CX996418 [+]aagggcgagagggggacaccatgtcagccagaggcaggacggcggaagatgtagccacagcttccagttaccgggagttccagcccgagcacatgcacct
ggagctgacagtagacacagaggctcgaatactgcggggctcccagcggctggacgtgcaggtgttgcgggagaccgaggagctgcgactggacactcac
cgctgcttgcgagtgctgggagctcggctggagaagggccagggggaacatgaggagttgtcgtgggaggaaaagcctttcacacgctatggcactatgg
tggttctccgtctgcccagcctactgccccccggagccagggttcgggtgatactcgattatgaggcagcggatagcccaggggtttgttggttggaccc
acagcagactgcaggcaagaagaagccttacatgtacacgcaaggccaggctgttttgaaccgctccttcttcccatgctttgacacaccggctgttaaa
tgcacctattctgccaatgtcaaggtgcctgaaggttttacagctgttatgagtgcaaataactctgaccgccaaggggatacttttaaattcgagatgg
cacaacctattccagcatatctggtggctctggtggttggtgacattgtatcagcagaggtaggaccaaggagccgtgtgtgggccgagccttgtcttat
agaagcagctaaaaaggagtatgatggagtaatagaggagttcctgaaagtgggggagaagctctttggtccttatgtatggggaaggtatgatgtacta
ttcatgccaccatctttcccatttggtggaatggagaacccctgcatcacatttgttaccccatgtctgctggctggtgatcgctcattggctgatggta
tcatacacg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: