XB-CLONE-3534827
Clone Name: IMAGE:7721152
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Gene:
rnpep
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX996554 [+]
agccagaggcaggacggcggaagatgtagccacagcttccagttaccgggagttccagcccgagcacatgcacctggagctgacagtagacacagaggct
cgaatactgcggggctcccagcggctggacgtgcaggtgttgcgggagaccgaggagctgcgactggacactcaccgctgcttgcgagtgctgggagctc
ggctggagaagggccagggggaacatgaggagttgtcgtgggaggaaaagcctttcacacgctatggcactatggtggttctccgtctgcccagcctact
gccccccggagccagggttcgggtgatactcgattatgaggcagcggatagcccaggggtttgttggttggacccacagcagactgcaggcaagaagaag
ccttacatgtacacgcaaggccaggctgttttgaaccgctccttcttcccatgctttgacacaccggctgttaaatgcacctattctgccaatgtcaagg
tgcctgaaggttttacagctgttatgagtgcaaataactctgaccgccaaggggatacttttaaattcgagatggcacaacctattccagcatatctggt
ggctctggtggttggtgacattgtatcagcagaggtaggaccaaggagccgtgtgtgggccgagccttgtcttatagaagcagctaanaaggagtatgat
ggagtaatagaggagttcctganagtgggggagaagctctttggtccttatgtatggggaaggtatgatgtactattcatgccaccatctttcccatttg
gtggaatggagaacccctgcatcacatttgttaccccatgtctgctggctggtgatcgctcatgggctgatgttatcata
3' EST: CX996553 [+]tggtttattgcattttcagtctgaacaagtgtggtacagtatgttgattacacttccattctgtagctggacaatgatctgcaatgctctatatgcgctc
tcattgtgcagatgtgcaactctatgttcctaggatcctctttacatagttctgcacattggcatgaagctgtggacaagtttgcgtaaaggtctcccta
gcgaggtcctgtgctgcctttgagccagcttgcatggcacggtataaaggtaaagtatacttctgcttcccctggcaatggagaaagtccctgactttat
gaaagtggagctgatagtcattcttcaatacaatctgggcccagcgcaaacgcagctcagcatttgtagcattggaaatctttggataatacttttccag
ttgttcgacatttccatcaggcagtggtgacaactcaagtactctatccaaaaagtagacaagctgataggtcctccatattgtgggatcaacttttgaa
atagtttcagtatcaagtggagtagaagaccagaggtttgctagttctgttgctggtttcataagtgcctctccaggggacaggtctgggaggaaaggag
gccatcctggagtgtttagccaatggtcaaattccagaccggatatcgtatccactccctttgctttcagttctggaaaatattctagataaaactcaag
ggcttcatcagcaagaatgctctggaacttgaacttattcacataggcctttaanaatgcatcaaacttggtctggtccccagtgaggtgggccaagtat
gaaacaaagcagaaccctttctcgtagggagtctcattataagtatcatctggatccacaccctgccatttgaaaagagaaagaaaagaggagaaatgtt
actatgcagcatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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