XB-CLONE-3534743
Clone Name: IMAGE:7721160
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Gene:
dop1b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX996611 [+]
agagagactagtctctctcccagtcttgttatttttcatacataaaggctttttagtcatctgtattcctggtgttggcaaaactgtcattaatggagga
atcttgataagtcggcaaaatggttctgttggccaatggcacgtgcagcactttaattgcttcagtccctgcagagcaaccatagtctattcccattatc
agcattatctgcccatcctgttattggaactattgttactgctaggggtgtagggcattttgagctttgtcgtgtatttgtactaacagtgcccaagagt
gtctcaccagggggcccaatttagttaatgagtagccaattgttgatgatgactgtggtagccacctggccagtattttgccggcccagccagtaagcgt
tctgcttgatgccagttaaagtaacatagacaagcaggccggtagtttatccccaaaaggtggcagccctagagctgaatgcctacttgctttcctgcaa
tccctgggcaatatgtgagttattctggatatacagcagtctgtctctgcattgctcagtattactgctcctctgtaacctgataatgagctacaggagt
ggcctgaacattctggaaacttctacccaccggcatttgtcaggtaccataactcgatccaataaatactgcagtattaatttacccccaaatatacatt
tcctaaaatcactccaaactagttatcaagtaaactaacagggtgcaccttttaattggcatctatgtgacatttagtacatatgtttctaggtaacaac
atggaagctaaatgcccttgttactaacaggcagtgagacttgtggggtgagaattgcatgga
3' EST: CX996610 [+]ttttttccatcttttattaacatttattactatacaaatctggaatattctgtaaacctgtaacccagtctctagaatgtttgcaatttgtaacaacgtt
cacttattccatcgttggcggagaggttgaagtggtttgcggattgctgtgagagtgattatagcgcactaacgaacgttattaatttacattcatatta
aaaacattataaagtcacattcagaacagtaaacacacagcacatcccacagactggagagtacggaatctgtacttcatatacagggatctcctgaaat
aaatcaacaatgaaaacaacttggtaaacatgtaaaggaaggaaccggtgttgtggttcgaacaatgggcaatcccagagctctcagctattggacagca
aagatctagaacccttattatttctgtctgtataacccaacaatgaatatactgtattattctctcagatggatgaatggaaactaaataaggttccata
cagagataaacaaattctgtcacaatctggaaccttttccattcactatacatttgattagtgccccttaaattgcccgcccctgaggagcctccagttt
gggaagaactattagacactcccctgacatattccatggattataatgttacattccaagtagaggaatgtgggaatggaacatgacacacagaaccttc
tctttctaaacctcacttatgattcaggccgatgctttccaatgtaattgaacattgctgcagtatctgatattctacttatcaaatgcaatgcgaacag
tatagaaagagaatttgatctgctcttactgngtgcaaaaagattgcagtnaaatatggaacaaggtaccagt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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