XB-CLONE-3534740
Clone Name: IMAGE:7721208
|
Gene:
gtf2ird1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX996613 [+]
gaaggcaaagacattctgtaaaaataataaaacaaaaatgcatcatagcataatgtcatatacatttgtttgtacaaaatttacatattttggatcatcc
aattgggagccaggatctagtagtttatggggccaggtaactgcatgtttacccctccataatccaacatgtacatgggccattgcacaagtgatatgtg
atgtgctgaggagatgggatccatgttggaggaggaggaggacgatgatgaagagttggaagaagctgaagataccgagttcccttctgacactcttttc
cgttttcgcttgggaccagagttagctttttttgttttggggggttcagtgcagatttcaggaaattgctctaactgttttattaccaccatctggattt
gatctcgtgctttgagaattttttcaagccgatgaaggtcataggtattagggtttctgaaagggatgtcatttggcagccctcggacttcaactgcatc
aggattatccctaataagtttgtacggaatcagcactggttggtccagacctagagctttaccatatttttcattaaagagttcctttacttgttctctg
agaataaccttttctcccaatctgttggcatcctcatcatcgggccttggaatgagtccttgaaatggccttgtaactgtgaatctgattttttcagcca
ccgctaatatcctttctagattctgtgacccaaaggtgcaaggctttcggaagggtatcccaggaggaaggccctctataatgactgaccctggattgtt
tttaatgcgtttgtaaggaacctggactggatactttatccctagggcttccccatattttttataaac
3' EST: CX996612 [+]ataaaatccncttttaaaagaccatgtacctatggtgtaccgaaattaaaaagaattttggaagaaaggcataatattcattttactattaaaagcatgt
ttgatcaacgaatatttgatgaaaccacaaagtccggctcttccagcccaggagaagaggcctgtagtgaaaaccagaaggcagctgctctggacatgct
tgggttctctgttggaagcagattggaaaaaagcagcatatcagatgaatgcgaaccagggacatcaagtgaaccaagtggaattaaacaaattaagttg
gaacctgaagacactgatataatacaagtcacagttccagaggcattagctgagtgtgtgaaaagtaatttgacttcagaggactcaagctatgtgctgg
acactggaaaagtttgtgatgatagaccctgtgatgaaaccaggaatgaagagaagcacctggatggaataggagatataataaggcagcttcggaaacg
agtagaaaatctctttaacagaaaatatgctaaggcaatcggagcctcaggacctgttaaagtgccatatgccaagttcttgatgtacccagaagatctc
tttgttctaggattgccagaaggtgtctcattccgacggccaaactgctttggaatcacaaaacttagaaggatactggaatgcagtaatggcattcagt
ttgttgtaaaaagacctgagttgatttctgaaggactgaaagactgtgttgtagacagccctggaagtctagggatcaatgataaaagtaatgaagctat
attagatgaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: