XB-CLONE-3534065
Clone Name: IMAGE:7721660
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Gene:
acta1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX997067 [+]
ggaaatcagcccgagcgagctggtggatcggctagagagcaatccctgcactgcctgaagaaaagcggagcccgagaaagctaccatcatgtgtgacgat
gaagagactaccgcacttgtgtgtgacaatggctccggacttgtaaaagctgggttcgctggagatgatgctccaagagcagtgtttccatctattgttg
gacgcccccgccaccagggtgtcatggttggtatgggtcaaaaggactcctatgtaggtgatgaagcacagagcaagagaggtattcttacactaaagta
tccaattgaacatggtattatcactaactgggatgatatggagaagatttggcatcacaccttctacaatgaactgcgtgtagcccctgaagagcatccc
accctgcttactgaggctccactgaaccctaaagctaaccgtgaaaagatgactcaaataatgtttgagactttcaacgtcccagctatgtatgttgcaa
tccaggcagtgctttctctatacgcctctggccgtacaactgggattgtgctcgactctggggatggtgtgacacacaatgtaccaatttatgaagggta
tgccttaccccatgccatcatgcgccttgatttggctggccgtgatcttactgactacttaatgaaaattctgacagaacgtggctactcttttgtcacc
actgctgaacgtgaaattgttcgtgacatcaaggaaaaactctgttatgtggctctggactttgagaatgaaatggccactgctgcctcctcatcttctc
t
3' EST: CX997066 [+]agttttttggggactggcataataaatttattattgttggaaattttcatgatgcttctgttagatcagtgcttcagttataaaacatcttgaaataaat
tacagtttatgtacactttatatatttcttgagtgacactgacaataaggcaacaatgacagaatgtgttacagatgaatttacattcgtgccttggtat
tttaacagcccgtattgaagaacattaatgcaggtctggtcattagtgcaaagatcaacataactatttagaagcatttacggtggacaatagagggacc
agcttcatcatattcttgttttgtaatccacatctgctggaaagttgaaagggaagccagaatggatccaccaatccagacagaatatttacgctcagga
ggagcaatgatcttgattttcattgtgctgggagccagggctgtaatctccttctgcatacggtcagcaatacctgggtacatggtggtacctccagaca
tcacattattagcatagagatcctttctaatgtcaatatcgcacttcataatgctgttgtatgttgtttcatgaataccagcagattccataccaataaa
tgatggttggaagagagtctctgggcaacggaaacgttcatttccaatggttataacttgaccatcaggcagttcatagctcttttctagagaagatgag
gaggcagcagtggccatttcattctcaaagtccagagccacataacagagtttttccttgatgtcacgaacaatttcacgttcagcagtggtgacaaaag
agtagccacgttctgtcagaattttcattaagtagtcagtaagatcacggccagccaaatcaggncgcatgatggca
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1678 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: heart | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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