XB-CLONE-3522560
Clone Name: IMAGE:7726099
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Gene:
ints15
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC123054 [+]
AGATGATCAGCGTATGGCGCTGCTGGGGAAGCTGACGTCTATGTCTGTGGCAGTGTGCAGGGTGCCTGTGTTGGAGTGTGCTGCATCTTGGTTGCAGCGC
ACCCCGGCTGTATATTGTGTTCGGTTGGCAAAGGCCCTTGTGGATGATTACTGTGGCCTAGTACCTGGCTCAGTCCAGACACTCAAGCAGATCATAAGTG
TCAGTCCTCGCTTCTGTTGCCAGTTCATCACTGCAGTCGCTGCTCTCTATGATATGTCCAGCGAGGAGCTCCTACCCCCTCATAATCTCCTGGAGATGAT
AGTTTCGTGGATTTTTGATGACCCTATGCTTATACTGATCACTTTCCTCAATACTCCTATTACTGCCAATGTGCCGCTTGGGTTCTTAGACTTTTCTCCT
CTCATGGGCCTGGTACGGTGGTGTGTGACGGCCCCTCTAGCATACAGGCGCAAACAGAAAGCCGCAGGGGGGCAGCTACCTGTAGAAAGCCAAGGCCTAT
ACTCCAAGCTGCATCTGAGTATGTTACAAGGTCTCATGGTCCTTCAGACTCACCTAACAGAAAAGAATCTTTATGGAAGACTGGGTCTCTTTCATTTTGA
CCAGCTTGTGCCTCTGGTGGAGGAATTGGGACAGTTGTGTGATGAGCTCAACCCATTAAATGCCGAGCAGGAGATGGAATTAGCACTAGATAGGCTTGCT
CAGGCTTTGCAAGTTTCCATGGCGACGGGGGCTTTACTTTGTGCCCGAGATGACCTGCGGACACTGTGCGCTAGGCTTCCCCATAATAACCTTATGCAGT
TGGTGATCTCTGGTCCTGTTCAGCCTCAGCCGGCTCACCCATCTCTAACCCCAGGCTATTACCCTCACATTCATACCCCGCCCCTGGGCTACCCCTCTCT
GCCTGCTCACCCAGCTCTGCCTGCTCACACGCCAATTCCTGCGCACCCCGCGGTGCCTGCTATCCCAGCAATTCCTGGGCACCCTGTGCAGACTTTCATG
CCTGGGATGGCCTTTCCATTCAGGCCTATGCGCTGAGTTACCAGCCTACAGAATGAGACTTTTACCTTTGCACTGCTGACAGAGAAGCGCCATGCAGAAG
GGCATCTGGGTATTTCTTAAATGTGCTCTGGTGCCTGTGAAACATCTGAACCTCATTTGGTGCTACAGGAACCACTTCCAACCACAAAAGAGAGTAAAAG
GACCAAAGTCATACAAACTTATTGCTGCTTGCTGCACACCATGCAGGCCTGGCTGAATTATATTAGTACCAGTAATACATTGTATGGATAGCAGTTCTTG
GACTTTTACTGTAGTCGAAGAAAGGGTTAATGACCTGCATCACCTCTTCCCTTTCCAGTGAGATAAGCGACCTGCAGCCCATCAGCTGCCCTCTAGTTCA
ACAACAAGGTGTTGGGCGTTCCCTGGAGTTCCTGCTAGTGAGGATGTTATTTGTGGCCCCAATTGATTGGCCATACCTCAGTGCAATAATGTCATGCCCC
TAAACTACTAGGTCAGGCCACTTTTTTCTGCTTCTTATTATAACTCGATAAGTCTATGGGAGATGATAAGGAAAGTTGTGGCCAGCTGAAACTGCAAGCT
TAGCTATTACCAGTTTCATAGGAGCCAGGCAAAATAAAGCAAAAGAAAAAAATATATACATTCAGCTCTTATTGTTTGTGTTTGACAAAGCCATAGAGAT
AGTAAAGATATAATGGCCCTTTAAAGGAGTAGTTCACCTTTAAATTAACTTTTAGTATGATAGAGGCAGTGATATTCTAATACAATTTGCAATTGGTCTT
TTTATTTTTTTATGGTTTTTCAGTTATTTAGAATTTTCTTCTGCAGCTCTCCAGTTTAGTATTTTAGAAGCTTTCTGGTTTCTATGGTGCAGTTTACCCT
AGCAACTAGGGATGCACCGAATCCACTATTTTTGGATTCAGCCGAACCCAGAATCTGGCAAAAAATGAATTCAGCTTCCATATTTATATGATAAAAACGC
GCGTGAGTTTAAAGATTCGGTTCGGCAAGGAGCGTGGATTCGGCCGAATCCGAACCAAATCTTGGATTTGGTGTATCCCTACTAGTAACCAGGCAGTTGT
TTGAATGAGGTGGGGATATGAATAGGAGAGGGCCTAGTAGAAAGATAACTAATAAAAAGCAACAATAACAATGAAATTGTAGCCTCACAGAGCAATAGTT
TTAGCTGCTGGGAAGATTCAGAAGATAAAGGCAAATAATAAAAAAAAACAATAAACATAAATAAATAATGAAGACCGATTGCAAAGTTGCTAAGAATGGG
ACATTCTACAACATACTAAAAGTTACATAAAGCTGAACCGCCTTTTAAAGATAATTTCTGAGTTTTTAATGCATTGCTCTGTTATATGCTGCGCCGCCGG
ATACCTGTTACCTCTGGTAGATATATAAAAATGGAGATCTTTTTATTTAATGGGAGAATATTAAAAGAACAGGTAACACCAGAAATGAAAATGATTTATT
CGCTTTGGTATGTACCCATGATTATAGCCACTGAACAAGTGTTTCTTTTTCTTGTTTTTATTTTTCATTGGCATTCATCTGGCTGCACACATGATGTATC
TGTGGGGCAAACAAGACAACCCCACTCATTCAGTCACATTGTTTTCCATTGAGCTAAAGGGCACACTCTATGTTCAGATTCACATTTTTTTAAAGAATGC
TATTCACTTCAGTTTCAGAGAGCACAAAGGGTTAAAGGCATTCCTGCAAATGCTATGTGTGCCGGTAGCTTTAGGGTAATGAGCACCCGGTTTATAAGCA
GCCTGAAAATGGCACCAAAGTGCTTGGAATTTACAGCGCAGATACAGTATAGGTCAATGTTGGTGCTTTACTTTCTACTGCTTACAAACGTGGCGCTCGT
AGTGCCCAATGTACCGTTACCCTTATTCATGACAACACATGAGCCCATACAAAGGCACACTTTATTTTATACGGATATGCTGTGATTGAATGCTGCAGAA
TTGATGTAATACAAACAGAACCTTTTCATTTTTTTATGCTGATGACAGAGGGTTGTGTACATTAGCCAGGAACACTCTACTGTTAAACCTGTATATTTGT
GTAAAATAACACTTTTTCTAATAAAACTTAAAATTTAGGTAAAAAAAAAAAAA
5' EST: DN004965 [+]agatgatcagcgtatggcgctgctggggaagctgacgtctatgtctgtggcagtgtgcagggtgcctgtgttggagtgtgctgcatcttggttgcagcgc
accccggctgtatattgtgttcggttggcaaaggcccttgtggatgattactgtggcctagtacctggctcagtccagacactcaagcagatcataagtg
tcagtcctcgcttctgttgccagttcatcactgcagtcgctgctctctatgatatgtccagcgaggagctcctaccccctcataatctcctggagatgat
agtttcgtggatttttgatgaccctatgcttatactgatcactttcctcaatactcctattactgccaatgtgccgcttgggttcttagacttttctcct
ctcatgggcctggtacggtggtgtgtgacggcccctctagcatacaggcgcaaacagaaagccgcaggggggcagctacctgtagaaagccaaggcctat
actccaagctgcatctgagtatgttacaaggtctcatggtccttcagactcacctaacagaaaagaatctttatggaagactgggtctctttcattttga
ccagcttgtgcctctggtggaggaattgggacagttgtgtgatgagctcaacccattaaatgccgagcaggagatggaattagcactagataggcttgct
caggctttgcaagtttccatggcgacgggggctttactttgtgcccgagatgacctgcggacactgtgcgctaggcttccccataataaccttatgcagt
tggtgatctctggtcctgttcagcctc
3' EST: DN004964 [+]gcgagaggttttttttttttttacctaaattttaagttttattagaaaaagtgttattttacacaaatatacaggtttaacagtagagtgttcctggcta
atgtacacaaccctctgtcatcagcataaaaaaatgaaaaggttctgtttgtattacatcaattctgcagcattcaatcacagcatatccgtataaaata
aagtgtgcctttgtatgggctcatgtgttgtcatgaataagggtaacggtacattgggcactacgagcgccacgtttgtaagcagtagaaagtaaagcac
caacattgacctatactgtatctgcgctgtaaattccaagcactttggtgccattttcaggctgcttataaaccgggtgctcattaccctaaagctaccg
gcacacatagcatttgcaggaatgcctttaaccctttgtgctctctgaaactgaagtgaatagcattctttaaaaaaatgtgaatctgaacatagagtgt
gccctttagctcaatggaaaacaatgtgactgaatgagtggggttgtcttgtttgccccacagatacatcatgtgtgcagccagatgaatgccaatgaaa
aataaaaacaagaaaaagaaacacttgttcagtggctataatcatgggtacataccaaagcgaataaatcattttcatttctggtgttacctgttctttt
aatattctcccattaaataaaaagatctccatttttatatatctaccagaggtaacaggtatccggcggcgcagcatataacagagcaatgcattaaaaa
ctcagaaattatctttaaaaggcggttcagctttatgtaacttttagtatgttgtagaatgt
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1678 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: heart | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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