XB-CLONE-3519159
Clone Name: IMAGE:7727684
|
Gene:
XB5886928
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DN007929 [+]
gcttttgctagttgccggatccatggcacaagaagacatggaaagaaatgtcttcctattccccactcggtctgtcattaattatgtggttctgacccca
atggtgactgagtctttagataaattgaccgtttgtttgaggatctatacaaatgacagaagatatgccctttttaacgtgggcacaacagagtcattaa
ttgaaatgtctgttgttttccagtccatgccatgttacacagacttgccatactgttcaaacgacatcttcatgaacactttgaaggtatctattcctgc
aaaggcagatgttctggactggaatcacatctgtgtgacctgggactcttatactggggttctacagctttgggttaatgggaaagtctcccctcgcaca
ataatgcaggaagccttttccgttgatcttcagaaaggttttgtcctgggtcagaagcgggattattattatcagaagtgggattcatacgtttcctttg
aaggggaaataagtgacgtccatatgtggaatagggttttgcctcctgagaccataaggaaggttctgctaaatagaaggaatattaatgggaatgtcat
tagctggaggtctctaaactacactctttatggtaatgtcactgtccaacccaaactccagtgtagatattgcggtaagcttggcttacaccatcctggg
ctgtctgaacaaaatatcaatatagagggcctagaaagtgaaaattagggccctngaaaaattcacttcttatggtgaaagttttctcttttggcactcc
cgttaaaccacatgattccggcctgtcagat
3' EST: DN007928 [+]agctttcatttaatgctgtttattggtaaatgagtatattgtaccattcccttaatatggccatatatcatgtcattgtgcaaatacaatatagtgttac
atggtgcaaaagataatcataaaatacacattatgataacacaaactgtggtgccagtgtggggtaatatgacccccttgggatgtgccacacaatggga
gaataaaatacaggttggtagatgctgatatcacggatagtttaaggatagggtggggtgggcatggggtattgggtcccagaatcaaaggggtctttaa
agggaaaccttagatagaagttcaaaagacaaattacattgtataagaattgtaagatctattaagtacatacatcaggcatggtcataatgcagccttc
cagttgttggtgggatgctgggagttatattttaacaaccggatgtctgtaattagaaatatttgatttatgcaatccccgcccacacatacaagatgga
ccagggatctcttcatccctaacatgagcaaaagatgagactagattagtagtgatgagcgaatctgtcccgtttcgccataaaattcgcgaaacggcaa
gaaaatttgcgaaatggcgaaaaatttgcaaaacgcatttgttgcgcgattttttgtcacccgcgtcttttttgtcaccagtggctatctttttgtcacc
acggctaattttcttgacgcccgcatttttttttaatgtgaccacgcccaatttgatgcgaccacgctcgttttgatgctgccacgcccattttga
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1678 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: heart | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: