XB-CLONE-3514848
Clone Name: IMAGE:7729614
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Gene:
chst12
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC123055 [+]
GTTAACCTGAATTAAGTCAAGCAGAAAAGAAAATCTTGGATCACAATGGCCAAGTCGAGGCTCTTCTGTCTGCTAGTGGCGTTGGGTTCAGTTTTCATGA
TTCTCTTCATCATTGTCTATTGGGACAATGTTGGGACAGCTAATCTGAATCTTCACACTTCCTTCAGCAAGTCTCTTCTTCCTCAGTCATCTGCAGAGCT
TTCGACTGCCGTTACAGTACCAAGAAACCGATTTGTTTCAGATGTAGATGCATTTTTAGATCACTTTCTTAATTTTAGCACAAAGAAAAGTGAATTTCAG
AGCACAAAAGCAGAAAAAATGCCACTACGAGGCCCAAGCAGCCTGGAGGAAAATGTCAGAGGATACGATTGGTCCACCAAAGAAAAATTAGAGGATGCAA
TTCTAGATCAGGAAATAATACAGCAGGAGAGAAAACGGAATTTGTTACAGTTTTGTGGCAACTCAAGCTTTAGTTTTCCGACAAAGGAACGTTCTTTTGA
TGACATCCCTAATCGGGAATTAGATCATTTAATAGTAGATGATCGTCATGGCATTATTTACTGCTACGTTCCAAAAGTTGCCTGCACCAATTGGAAGCGA
GTGATGATTGTGCTAAGTGAGAGCCTCCTGGACAAGAAGGGAGTTCCGTACCAAGATCCCTTACTAATTCCTCGTGAGGATGTCCACAATACAAGTAGTC
ACCTAACCTTTAACAAATTTTGGCGCCGTTATGGTAAATTTTCTCGACACATGATGAAGATTAAGTTAAAGAAATACACCAAGTTTCTTTTTGTTCGTGA
TCCTTTTGTCCGTCTCATCTCTGCATTCCGCAGCAAGTTTGAGCTTGAAAATGAAGACTTCTACCGCAGCTTTGCAGTGCCTATTTTGACTCGATTCTCC
AATAGAACCAGCGTTCCTGACACAGTCGGGGAAGCGTTCTCCTCTGGCGCAATGCCATCTTTTTCTCAATTTATGCAATATCTTCTGGATCCACAGACAG
AAGAGCAGAGGCCGTTCAATGAACATTGGAGGCAGGTGTATCGCCTATGCCACCCATGCCAAATTGAATATGATTTTATTGGCAAGCTGGAAACTCTCAG
TGAAGATGCAGCACTTCTTTTAAGACAATTAAACTTAGAGTCACTCTTCCAGTTCCCTCCCAGTTACAGAAACAGGACTGCGAGCAGCTGGGAGGATGAC
TGGTATTCAAAACTTCCTGTAGCTTGGAGAAAGAAACTGTACAAATTGTACGAGGCTGATTTTGTTCTCTTTGGCTACCCCAAACCAGAAAACCTGCTTA
GTGTATAAAACAGACTTGCTGAAATACCAAAAACCGAACCATAGTTTGCTGCCTTTCATAAAGATTTCCAGGGTTGGTACAATTTTATAGGTTTTTTTTT
TTAATTTCTTTCTATTGTATGTGGAAGCATGGGTTATAGTTTACAGGAAAAGTCAACCTCCTTAGCAGAATGAAGTTGGAGTAATTTAATGGGGAACTAA
AAACCCGAAGATGCACTTGCTCTGGATAATGTACTTGTGTAAAGGTTGTACATGTTTACTATTGCACACCAGCTCTTTGGAATACAAGTATTACCCCCTG
CATACTTGTAGGTTGCCCCAAGCCAAAACAAATATCAAACATAAAACAAGCATTTTTGTCACATGCACATTGAGCGTTCTTATATGGTTCACTGATATAA
CTCCCTGTTAGTGAAGCACAAATGTAGTGTAATGCCAAAAGGTATTAGTTGGTGTGAGGTTAGTATGTGTACTACCACTATAAAATGAGTACTTTGGTTA
AGAAAATGTTTCTAAAGTGTTTTAGTTACCCTTCAAAATTCCTTTTTTTTTGTATAAAATTACTGCTTCTTAGGAAACCAGTAATGCTCCTGCAGTCCTG
CTTTTCAAAATAGGGAGCCTTTGGGTAACCTAGTAACTTTTTAACCATGGGTATCGCCTCCATCTCCTCCACGTTAAAGCATATGTTTAACCTGAAAGTT
CTGTGCTACACAGCAGTCTGCTTACAGCGGCAGGACACTGAAGTGTTGTGGGCAGTGTACATTATAGAAGAGCAGTATTTTTATTCTTCTTTTTTTTTTA
ATGTTTGTAAAAAGATACCCTTGTCATTCTAACGTCTCAGGAGGAGTTCTTTTAAATGCTTTTTACTATATAAATATTATTTTCTAAATTAATATATTAT
TTAAAGTATTTAATACAGGAACTTGGTCAAGTAAAGGCTTTGCTGTTCAAGCAAAAAAAAAAAAAAAAAA
5' EST: DN010907 [+]taacctgaattaagtcaagcagaaaagaaaatcttggatcacaatggccaagtcgaggctcttctgtctgctagtggcgttgggttcagttttcatgatt
ctcttcatcattgtctattgggacaatgttgggacagctaatctgaatcttcacacttccttcagcaagtctcttcttcctcagtcatctgcagagcttt
cgactgccgttacagtaccaagaaaccgatttgtttcagatgtagatgcatttttagatcactttcttaattttagcacaaagaaaagtgaatttcagag
cacaaaagcagaaaaaatgccactacgaggcccaagcagcctggaggaaaatgtcagaggatacgattggtccaccaaagaaaaattagaggatgcaatt
ctagatcaggaaataatacagcaggagagaaaacggaatttgttacagttttgtggcaactcaagctttagttttccgacaaaggaacgttcttttgatg
acatccctaatcgggaattagatcatttaatagtagatgatcgtcatggcattatttactgctacgttccaaaagttgcctgcaccaattggaagcgagt
gatgattgtgctaagtgagagcctcctggacaagaagggagttccgtaccaagatcccttactaattcctcgtgaggatgtccacaatacaagtagtcac
ctaacctttaacaaattttggcgccgttatggtaaattttctcgacacatgatgaagattaagttaaagaaatacaccaagtttctttttgttcgtgatc
cttttgtccgtctcatctctgcattccgcagncagtttgagcttgaaaatgaagacttctaccgcagctttgcagtgcctattttgactcgaatc
3' EST: DN010906 [+]gcttgaacagcaaagcctttacttgaccaagttcctgtattaaatactttaaataatatattaatttagaaaataatatttatatagtaaaaagcattta
aaagaactcctcctgagacgttagaatgacaagggtatctttttacaaacattaaaaaaaaaagaagaataaaaatactgctcttctataatgtacactg
cccacaacacttcagtgtcctgccgctgtaagcagactgctgtgtagcacagaactttcaggttaaacatatgctttaacgtggaggagatggaggcgat
acccatggttaaaaagttactaggttacccaaaggctccctattttgaaaagcaggactgcaggagcattactggtttcctaagaagcagtaattttata
caaaaaaaaaggaattttgaagggtaactaaaacactttagaaacattttcttaaccaaagtactcattttatagtggtagtacacatactaacctcaca
ccaactaataccttttggcattacactacatttgtgcttcactaacagggagttatatcagtgaaccatataagaacgctcaatgtgcatgtgacaaaaa
tgcttgttttatgtttgatatttgttttggcttggggcaacctacaagtatgcagggggtaatacttgtattccaaagagctggtgtgcaatagtaaaca
tgtacaacctttacacaagtacattatccagagcaagtgcatcttcgggtttttagttccccattaaattactccaacttcattctgctaaggaggttga
cttttcctgtaaactataacccatgcttccncatacaatagaaagaaattaaaaaaaaaacctataaattgtaccaaccctggaaatctttatgaaaggc
agcaaactatggttc
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1678 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: heart | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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