XB-CLONE-3494793
Clone Name: IMAGE:7737327
|
Gene:
ass1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DN024622 [+]
ctcaacccatcgatccactgctgtttcattgacacccgacaagatgtcccagagtaaaggcatggtggtccttgcatacagtggggggctggacacgtcc
tgcattctcgtgtggctgaaggagcagggttatgatgttatcgcttacctggcaaatattgggcagaatgaagattttgaggcggcccggaaaaaggctg
tgaatcttggtgcaaaaaaggtgtatatagaagacatgagacagcagtttgttgaagaatatatctggccggcagtacaggccaatgctatttatgagga
tcgctatctactgggcacctcactggcacgtccctgtattgccaaaaagcaagtggaaattgccaagaaagaagcagcagagtatgtgtcccacggtgcc
accggaaagggaaatgatcagattcgtttcgaactcacatgttacgcgctgtaccccgaagtcaagatcattgctccatggaggatgcccgagttctata
accgattccgagggcgcagtgacctgatggagtatgccaagaaacacaacatccctgtcccagtgacccccaaggacccttggagtatggacgagaacat
catgcacatcagttacgagggtggaattctagaaaaccccaagaaccatgctccacctggactgtatttaaagacaaagaaccccgcaacgtctccaaat
gagccagacata
3' EST: DN024621 [+]tatgtctggctcatttggagacgttgcggggttctttgtctttaaatacagtccaggtggagcatggttcttggggttttctagaattccaccctcgtaa
ctgatgtgcatgatgttctcgtccatactccaagggtccttgggggtcactgggacagggatgttgtgtttcttggcatactccatcaggtcactgcgcc
ctcggaatcggttatagaactcgggcatcctccatggagcaatgatcttgacttcggggtacagcgcgtaacatgtgagttcgaaacgaatctgatcatt
tccctttccggtggcaccgtgggacacatactctgctgcttctttcttggcaatttccacttgctttttggcaatacagggacgtgccagtgaggtgccc
agtagatagcgatcctcataaatagcattggcctgtactgccggccagatatattcttcaacaaactgctgtctcatgtcttctatatacaccttttttg
caccaagattcacagcctttttccgggccgcctcaaaatcttcattctgcccaatatttgccaggtaagcgataacatcataaccctgctccttcagcca
cacgagaatgcaggacgtgtccagccccccactgtatgcaaggaccaccatgcctttactctgggacatcttgtcgggtgtcaatgaaacagcagtggat
cgatgggttgag
Library:
Description: The library was prepared by oligo-dT priming (5'-GAGAGAGAGAGAGAGAGAGACTAGTCTCGAGTTTTTTTTTTTTTTTTTT-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: liver | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: