XB-CLONE-3494516
Clone Name: IMAGE:7737402
|
Gene:
fmr1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DN024815 [+]
ccgaggcctgataaggagaagggatatcagagtgaagaccttagtggcactgggagaggaagcagaccttacaataacagaggccgcagtcgaaggggca
caggttatgcctctgacataaggtatggagatccagattacagaaagaccacttatccggaataccccaggtcccaagcattctggataaaaggcacaaa
ttcagaagcatcaaatgcctcagagacagaatcagaccaccgagatgagctcagtgattggtcattggctccagctgaggatgacagagataattatcat
cgaaggggggatggacgcagacgagggggacctcgaggtcaaggcatgagaggtcgtggaggatttaaaggaaacgatgaccaaccacgaccggacaaca
ggcaacgcaattcaagggagacaaaagcaaggacatcagatggctctttacagatccgtattgactgcaataatgaaaggagtgtacatactaaaaccct
gcaaaacgcatctgttgaaggcagccgactgcgcactggcaaagaccgcgttcaaaagaaagagaagagcgaagtggtggacggaccacaagtggtggtt
aatggcataccataaccctatatgttgcaaataaaccctacatagtgcaaacaaaataccaagaaatacaaaattctcatttcacaatagagaaacctgt
taggccaaagacaaatgggcaagacaatgcagggcctacatgaattgctcctgttagccttattttgttagtgttggctgtaagcagttttcagaactca
gacattggaaacctatttgcattcaatatcaaaaccttcgaggcttttcagtacatattgcacttgactttaaaatttaaaaagtattttttgttaaa
3' EST: DN024814 [+]cgagaggcttttaaacaaaaaatacttttttaaatttttaagtcaagtgcaatatgtacttgaaagcctcgaaggttttgatattgaatgcaaataggtt
tccaatgtctgagttctgaaaactgcttacagccaacactaacaaaataaggctaacaggagcaattcatgtaggccctgcattgtcttgcccatttgtc
tttggcctaacaggtttctctattgtgaaatgagaattttgtatttcttggtattttgtttgcactatgtagggtttatttgcaacatatagggttatgg
tatgccattaaccaccacttgtggtccgtccaccacttcgctcttctctttcttttgaacgcggtctttgccagtgcgcagtcggctgccttcaacagat
gcgttttgcagggttttagtatgtacactcctttcattattgcagtcaatacggatctgtaaagagccatctgatgtccttgcttttgtctcccttgaat
tgcgttgcctgttgtccggtcgtggttggtcatcgtttcctttaaatcctccacgacctctcatgccttgacctcgaggtccccctcgtctgcgtccatc
cccccttcgatgataattatctctgtcatcctcagctggagccaatgaccaatcactgagctcatctcggtggtctgattctgtctctgaggcatttgat
gcttctgaatttgtgccttttatccagaatgcttgggacctggggtattccggataagtggtctttctgtaatctggatctccataccttatgtcagagg
cataacctgtgccccttcgactgcgggctctgttattgtaaggtctgcttcctctccagtgccactaaggtcttcactctgatatcccttctccttatca
ggcct
Library:
Description: The library was prepared by oligo-dT priming (5'-GAGAGAGAGAGAGAGAGAGACTAGTCTCGAGTTTTTTTTTTTTTTTTTT-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: liver | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: