XB-CLONE-3492282
Clone Name: IMAGE:7737948
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Gene:
bbs5
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Species: Xenopus tropicalis
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Sequence:
5' EST: DN026376 [+]
tttgttaaagagaaaaggaatatcaaatttaaaatgattatttgtttaaaatggagtctgtgggagatgcccttcctgtaattccaggctttctgggtat
tgggtttctggtgagggatcccatgcctgtactgtagctaatatgtttctgtgcttccaacaaccagaatgaaaacaatgcagaatatcttcattctgag
aaattgcagccacacaatcagccttgcctcaaaaccagataattagagagtagccctcacttgaggatcagcatttgtttcatctccatatctcaggctt
actgtgcatctggaatgagcattttaattgcatagacgcagccacaaattcagagccatgtatcccatacagactaaaggaatcttcttagctattacca
tctcagaataggggagaatggctgcagcagttcctggatgcacagtaaggggcatatttattatgctgtgtaaaactaattcgccgaaaaaacggagtaa
aaagatgtgtaaaataaatggaaaagatcgccatctgaacgccggatattaagccattattttccataagttccggtggaagaaaaaacgcgtaaaagaa
ttacgccgattttgcgctgtttttacaccatttttacacagtgaagcctggcgatgtgtgccgaattttctcgcagttttttacacagcataataaatat
gcccctaagtgtaaagaacataccaatggataccatgtatctgcatttatctttttttttgaggcattggcaggactaaata
3' EST: DN026375 [+]atgaagcaattctgtatttatttatgcaagcacatcagttcaaacacagagtaccctgctttgcagtacagcttttgtattgagcaagatggttggaaca
gagcaaactttccaaaaaaaaaaaaagacagtattctggaatgattgagagcaagaggaaaaatgtagtagtctgagccccctgaggaatggcatatgga
tggcatttatagcatgtctgtgtgaggcttacacaagccctacccctccccttgcttatggtaccagtgataaagtaacgcattttctttatatatatat
atatatcacaaaaaataattaaggcataattacatggcaaattatctcctattttaaattaaataaattcctttcatactttcactttttcaatatatat
atatatttataattcaacatgtctttggataacaaattctgtaggcaacaatttggtttttaaagggcttgtttagtctggcaaatatgcccttacaaat
cagagaagtttggtcccactgatctttatggattagaccatactgctaattaatactgcagtgagcgatagatcaccctgctatttaatactgcagcgag
taatagattacactgctaattaatagtgcagcaagtagaagagatttcaattgtctgtatgattgttgcagcttttgtctgttgcaggaaagttgcagtt
tgcctttcatggtagaaaccacaaagttggatcagactgttgcagttaagctgcaatggatgtacagacttttaacaattaacaatcattt
Library:
Description: The library was prepared by oligo-dT priming (5'-GAGAGAGAGAGAGAGAGAGACTAGTCTCGAGTTTTTTTTTTTTTTTTTT-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: liver | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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