XB-CLONE-3488598
Clone Name: IMAGE:7739529
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Gene:
ppp1r3c
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC155399 [+]
CTCCTATCCAGAATGATACAGATTTTGGAACCCCGCTCTCTCCCACGATCCATAATGCCTGTAGATGTGGCAGTTAGGATATGCCTAGCACATTCTCCAC
CGCTAAAAAAATTTCTCAGTCCCCCTGATTGCAGGGGAAGAAATTTTGTGAACCGATATAAGCCACTAAGACCATGCCTCAGTCTGAAAAAGGAATCTGA
TTTCAGGAGCAACGAGTGGAACCGCTCAAAGTCTAGAAACAAAAAGAAGGTCATCTTTGCTGATTCCAAAGGACTTTCCTTAACTTCGGTGCATGTCTTC
TCAGAGTTTAAGGATGAATCTTCATTAGATCTTCAGTTTGACCTGATAGACCTAGAAGATATAACAGCCAGTTTAAAGCTGCATGAAGAGAAGAACTTGA
TACTGGGATTCACTCAGCCTTCAGCTGATTATCTGCAGTTTCGCAATCGCCTTCAGAAGAGTTTAGTCTGCCTAGAAAATTGCAGCCTTCAGGAAAGATC
TGTTGCAGGTACTGTCAAAGTAAAAAACCTGAGCTACAAGAAATCTGTCAAGATTCGTATCACCTTCAACACCTGGAAATCATTTGTAGATGTTGATTGT
GTCTACATGAACAACGTCTATGGAAGCACTGACAGTGACACATTTTCCTTCGTCATTGACATACCCCCCAACATTCCCAGTCATGAGAAAATCGAATTTT
GTATATCCTATGAAAGTGAAGGGCAGGTCTTCTGGGACAACAATGATGGACTGAACTACTCAGTGACACGTGCAGAATGGAAATCAGATGGTGTGCACAC
ACCTACCCCAACTAAAACAGAGATTGCATCATATGACTATAAGAAGAAACCACAAAACAATGACTTTGATCAGTTTGGCAGCCCCAGAACATCTGCTGGT
CTGTACCCCGAATGGCAAAGTTGGGGTAAAATAGGAGGATCACCTTACTGGTGAAGAACATTGTTGATGTTCTGCTTTATTCATTCTCATCGAGACATCT
CGGTGCTGATGAGACATCATTTGCAAACAAACAAATGAAATTCTTCAAATAAAGTAACAGTGTAAGGAAAGCTGATAGCTATATGTTCACAGCCATAGCT
TAGGACAGTACGCTTTTCACAAGCACGTTGTCAAAACACCATATGTACTGCCTTTGTCCACCATAGAAATAGAGCGCTCAGTGCTGATGCCAGCTGAATC
TCTGGCGTTAGTCATATACACAGAATATACATTTGTGTAAGGCTAATATGATGAGCTATAAACACAGCAGCTTGTAGTAACCCATATGAGGATAGTTCTT
ACAAAGAGGGCATCGGATAGAATTCAGGACACCCTAAGGATATGACAATGAAGATTTTCATTCATCCAGGTCATGGTATATCTATTATAAGTAAATACTT
AGACACCCTAAGGAGTTTTGCAGATATGTGTTTTAGGATGAATTTATGGTACGAAATGGCTAACGATACAACCCCTGAAGGTTGAAAAGCTGAAAATACA
AAGAGACTGTCAATGCAATACTTACATAATGATGTTTGAGTTGCTATCATGTATTTCTAGTGACCTGCCAGTAAGCGCGAAACGCACCATACACTTCGGA
AAGTAGTGTTAGGTTTCATTTTAAAAGCTGGACTTTTAAAACCTTTCACAGACTCAGTTTAAATAATGTAAGATCATACAAGGTGGCTCAGAGATGTGCA
ACTGGTATCCTTGTAGCTGTTGTTATACTATAATTTCCACTAAATAATACAACAGCAACCAATGTATCACACTTTGGACATCCCTATCAAAATTTTTATG
GATGTGACTAAAGAATGAATAATTTTGCATTGGAGGCAAGAGTACAGAAAGTCTAAATGCACTAACCAATCCAGTGCACTTTTTTTGGTAAGGGAGGGCC
CTGCAATGTTGCTTTAAGCTATCCCAACAAATCTGCTTTGATGTTGTAAAAAGTGCCTTTTCTAAAAGATTGCCAAAAACCCCTTTAATACAGAATTGTA
GGTTTCCTGAATATAAAATAGTCAGTAGCAATGCTGTGCCATTTAGTAAACTGCAAAACCAAACTGCATAGTTACACTACATCCCTTATATATTGTCAAT
TTCTGAACTATTTCCCTTCCCACTCTTGATCCCACTTGATGCTAGAGGTGTGTACAACATAGTGCCATGCAATGCATCTGTCCACTCTGGCCCACTGGGC
TCTGGCAAACCTAAAACAAAGGATGTATTATATTGGAAGATTACCAGATGTCCAACATTTTAGTGTTATGTGTAAATAAGGAGCAACCTTTCAGGATAAT
AATACCCTCATTCTCCCTGCCCTGTAAAAAAAAATGCACTTGTTTGTAAGCAATCTGTAATATTCCCTGTCTTCTATGTTTTTTGAGATAATTACAGAAG
TCAGAAAATTCAATTTCCTTCATATTGAAGGTAACGCAAATCGCTTTTGCACCTTTATATGTGAATTTGGTATTTGATAGAATTGTAGGTTCCTTAAAGG
ATGAAATGATTGTAGTCGATATTTTTGCACATCAGATAGTCAAACCTTATTTTCCTTCTGAAGAGTAATGACAGCTTTTTATGAAATACCTTTATGGCGC
AGAGAATTATGTTTTATTTTCTCTATTCTTCCTTTTTCTCTCAATAGAAACTCTTGCACTTTTTACATGAAATAGCTGAGCAGTGTATTTTTCTATTTTG
TAACTGGCTTAAATGTGTATTTTTTGATGTACAGTACCTTCGTAAGCTGACAATATTGTTTTTTGGTGTCAGAATGAAAAAAATGAGCAGCGTTCTTGAC
TGTGAAATGAACTGTGATGCTTTTTAAAACTGATAAAGATTAATTGGATATTGCTGTATCCAATGTGTGAAACGAAATCAGCTTTAAGGAATGATAAAGG
TCACTTTATTAGCACAGTATGTCAAATGTTGAACTTTAGAGTGATGAGAGAGTGAGTTCTAACCCAACAGTCTGTTTTCCTACAATGTTTTATCACTGTA
5' EST: DN028860 [+]ctcctatccagaatgatacagattttggaaccccgctctctcccacgatccataatgcctgtagatgtggcagttaggatatgcctagcacattctccac
cgctaaaaaaatttctcagtccccctgattgcaggggaagaaattttgtgaaccgatataagccactaagaccatgcctcagtctgaaaaaggaatctga
tttcaggagcaacgagtggaaccgctcaaagtctagaaacaaaaagaaggtcatctttgctgattccaaaggactttccttaacttcggtgcatgtcttc
tcagagtttaaggatgaatcttcattagatcttcagtttgacctgatagacctagaagatataacagccagtttaaagctgcatgaagagaagaacttga
tactgggattcactcagccttcagctgattatctgcagtttcgcaatcgccttcagaagagtttagtctgcctagaaaattgcagccttcaggaaagatc
tgttgcaggtactgtcaaagtaaaaaacctgagctacaagaaatctgtcaagattcgtatcaccttcaacacctggaaatcatttgtagatgttgattgt
gtctacatgaacaacgtctatggaagcactgacagtgacacattttccttcgtcattgacataccccccaacattcccagtcatgagaaaatcgaatttt
gtatatcctatgaaagtgaagggcaggtcttctgggacaacaatgatggactgaactactcagtgacacgtgcagaatggaaatcagatggtgtgcacac
acct
3' EST: DN028859 [+]tacagtgataaaacattgtaggaaaacagactgttgggttagaactcactctctcatcactctaaagttcaacatttgacatactgtgctaataaagtga
cctttatcattccttaaagctgatttcgtttcacacattggatacagcaatatccaattaatctttatcagttttaaaaagcatcacagttcatttcaca
gtcaagaacgctgctcatttttttcattctgacaccaaaaaacaatattgtcagcttacgaaggtactgtacatcaaaaaatacacatttaagccagtta
caaaatagaaaaatacactgctcagctatttcatgtaaaaagtgcaagagtttctattgagagaaaaaggaagaatagagaaaataaaacataattctct
gcgccataaaggtatttcataaaaagctgtcattactcttcagaaggaaaataaggtttgactatctgatgtgcaaaaatatcgactacaatcatttcat
cctttaaggaacctacaattctatcaaataccaaattcacatataaaggtgcaaaagcgatttgcgttaccttcaatatgaaggaaattgaattttctga
cttctgtaattatctcaaaaaacatagaagacagggaatattacagattgcttacaaacaagtgcatttttttttacagggcagggagaatgagggtatt
attatcctgaaaggttgctccttatttacacataacactaaaatgttggacatctggtaatcttccaatataatacatcctttgttttaggtttgccaga
gcccagtgggccagagtggacagatgcattgcatggcactatgttgtacacacctctagcatc
Library:
Description: The library was prepared by oligo-dT priming (5'-GAGAGAGAGAGAGAGAGAGACTAGTCTCGAGTTTTTTTTTTTTTTTTTT-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: liver | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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