XB-CLONE-3475290
Clone Name: IMAGE:7744421
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Gene:
mat1a
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC155491 [+]
CCAAAACAAAATGAATGGACCAGTTGATTATATACAGGAACATGCAGTGGAAGACGTAGGAGCATTTATGTTTACATCAGAGTCTGTTGGAGAAGGTCAT
CCTGATAAGATCTGTGACCAGATCAGTGATGCTGTTCTTGATGCCCATTTGAGTCAGGATCCAGATGCCAAAGTGGCTTGTGAGACAGTTTGTAAAACTG
GGATGGTTTTGCTTTGTGGAGAAATAACATCCAGAGCTGTTGTGGATTATCAAAAAATAGTTCGAGACACAATAAAGTACATTGGATACGATGACTCTGA
AAAAGGCTTTGACTATAAGACTTGCAATGTACTGGTAGCTCTTGAACAACAGTCTCCTGATATTGCACAAGGAGTTCATTTGGATCGTACAGAAGAGGAT
ATTGGAGCAGGGGATCAGGGATTAATGTTTGGTTATGCAACAGATGAGACAGAGGAGTGCATGCCCTTAACTATAGTTCTTGCTCACAAACTGAATTCCA
AGTTGGCTGAGTTGAGACGCAATGGGGTTCTGCCATGGCTCCGACCAGATTCCAAAACACAGGTGACAGTGAAATACATTCAGAAGAATGGAGCAGTCAT
TCCTGTACGTGTTCACACTATTGTGATTTCAGTGCAGCATGATGAAACCATCTCTTTGTCTGACATGCAAGAGGCCCTAAAGGAACATGTCATTAAGGCT
GTTGTCCCTGCAAAATACCTGGATGAGAAAACTGTTTACCACCTTCAACCAAGTGGGCGTTTTGTCATTGGTGGACCACAGGGGGATGCAGGTGTCACAG
GCCGTAAGATTATTGTGGACACCTATGGTGGATGGGGTGCTCATGGAGGTGGTGCATTTTCAGGGAAAGACTACACTAAAGTTGACCGTTCAGCCGCCTA
TGCTGCCCGTTGGGTAGCAAAATCACTGGTGCATGCAAAGCTCTGCCATCGTGTTCTGGTTCAGGTATCTTATGCTATTGGTGTGGCCTATCCACTGTCA
GTTTCTCTATTCACCTATGGTACCTCAGAGAAGACTGAAAAGGAATTACTGGATATTGTAAACGCAAACTTTGATCTGCGACCTGGAGTAATTGTCAGGG
ACCTTGACTTGAAGAAGCCGTTATACCAGAAAACTGCATGTTATGGTCATTTTGGCAGAGAGGACTTCCCGTGGGAGGTTCCAAAGGAGCTTTTTTTCTA
ATGGTGAAAAATGTTTTTGTACCATATTACACACAAATGCAACTGAAGCAATCCTGATTTATAAATTCCACATGACTGTCTGTGGCAGAGTGCTTGCAGC
TGTGACTCTCGAGTCAAAATGGAATGCAAATACAGCTATCAAACAGAATCAGAGTGGATACAAACAAATGATCTTAAGACCCAAACTAATTCCTTCAAAT
AAAAACTATATGCCCTCTACCTCCTTTTTTCAAAATCTGAAAAAAAAAAAAAAAAAA
5' EST: DN038081 [+]ccaaaacaaaatgaatggaccagttgattatatacaggaacatgcagtggaagacgtaggagcatttatgtttacatcagagtctgttggagaaggtcat
cctgataagatctgtgaccagatcagtgatgctgttcttgatgcccatttgagtcaggatccagatgccaaagtggcttgtgagacagtttgtaaaactg
ggatggttttgctttgtggagaaataacatccagagctgttgtggattatcaaaaaatagttcgagacacaataaagtacattggatacgatgactctga
aaaaggctttgactataagacttgcaatgtactggtagctcttgaacaacagtctcctgatattgcacaaggagttcatttggatcgtacagaagaggat
attggagcaggggatcagggattaatgtttggttatgcaacagatgagacagaggagtgcatgcccttaactatagttcttgctcacaaactgaattcca
agttggctgagttgagacgcaatggggttctgccatggctccgaccagattccaaaacacaggtgacagtgaaatacattcagaagaatggagcagtcat
tcctgtacgtgttcacactattgtgatttcagtgcagcatgatgaaaccatctctttgtctgacatgcaagaggccctaaaggaacatgtcattaaggct
gttgtccctgcaaaatacctggatgagaaaactgtttaccaccttcaaccaagtgggcgttttgtcattggtggacc
3' EST: DN038080 [+]cagattttgaaaaaaggaggtagagggcatatagtttttatttgaaggaattagtttgggtcttaagatcatttgtttgtatccactctgattctgtttg
atagctgtatttgcattccattttgactcgagagtcacagctgcaagcactctgccacagacagtcatgtggaatttataaatcaggattgcttcagttg
catttgtgtgtaatatggtacaaaaacatttttcaccattagaaaaaaagctcctttggaacctcccacgggaagtcctctctgccaaaatgaccataac
atgcagttttctggtataacggcttcttcaagtcaaggtccctgacaattactccaggtcgcagatcaaagtttgcgtttacaatatccagtaattcctt
ttcagtcttctctgaggtaccataggtgaatagagaaactgacagtggataggccacaccaatagcataagatacctgaaccagaacacgatggcagagc
tttgcatgcaccagtgattttgctacccaacgggcagcataggcggctgaacggtcaactttagtgtagtctttccctgaaaatgcaccacctccatgag
caccccatccaccataggtgtccacaataatcttacggcctgtgacacctgcatccccctgtggtccaccaatgacaaaacgcccacttggttgaaggtg
gtaaacagttttctcatccaggtattttgcagggacaacagccttaatgacatgttcctttagggcctcttgcatgtcagacaaagagatggtttcatca
tgctgcactgaaatcacaat
Library:
Description: The library was prepared by oligo-dT priming (5'-GAGAGAGAGAGAGAGAGAGACTAGTCTCGAGTTTTTTTTTTTTTTTTTT-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: liver | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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